Literature DB >> 31718647

Correction to: A new primer construction technique that effectively increases amplification of rare mutant templates in samples.

Jr-Kai Huang1, Ling Fan2, Tao-Yeuan Wang1, Pao-Shu Wu3,4.   

Abstract

Following publication of the original article [1], the author informed us that the legend for Fig. 2 was incorrect.

Entities:  

Year:  2019        PMID: 31718647      PMCID: PMC6849285          DOI: 10.1186/s12896-019-0562-2

Source DB:  PubMed          Journal:  BMC Biotechnol        ISSN: 1472-6750            Impact factor:   2.563


Correction to: BMC Biotechnol (2019) 19:62 https://doi.org/10.1186/s12896-019-0555-1 Following publication of the original article [1], the author informed us that the legend for Fig. 2 was incorrect. Fig. 2 legend has been corrected in the original article. The correct legend for Fig. 2 is given below: Mutant sequence enrichment using stuntmer PCR. The abilities of stuntmers to detect four different mutation scenarios (single nucleotide mutation, two different single nucleotide mutations, and a deletion mutation) were tested under three different sample conditions (only wild-type, 90:10 ratio of wild-type:mutant, and 99:1 ratio of wild-type:mutant). In all cases, the stuntmers were able to detect the mutations even when the mutant templates were present in only 1% of the tested samples. The wild-type template was completely inhibited in the T790 M point mutation (a) and exon 19 deletion mutation (b). The arrow indicates where the deletion mutation occurred
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1.  A new primer construction technique that effectively increases amplification of rare mutant templates in samples.

Authors:  Jr-Kai Huang; Ling Fan; Tao-Yeuan Wang; Pao-Shu Wu
Journal:  BMC Biotechnol       Date:  2019-08-23       Impact factor: 2.563

  1 in total

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