Nabil Saadaoui1, Asma Weslati1, Taha Barkaoui2, Ikram Khemiri3, Wafa Gadacha1, Abdelaziz Souli1, Moncef Mokni4, Mounira Harbi5, Mossadok Ben-Attia1. 1. Environment Biomonitoring Laboratory (LR01/ES14), Department of Life Sciences, Bizerta Faculty of Sciences, University of Carthage, Zarzouna, Tunisia. 2. Biochemistry and Molecular Biology, Research Unit (UR17ES20), Faculty of Sciences of Bizerta, Carthage University, Zarzouna, Tunisia. 3. Department of Biology, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia. 4. Department of Anatomy and Pathological Cytology, Farhat Hached University Hospital, Sousse University, Sousse, Tunisia. 5. Laboratory of Horticulture, National Institute of Agricultural Research of Tunisia, INRAT, Ariana, Tunisia.
Abstract
Context: Vitis vinifera leaves are traditionally used in Tunisian folk medicine to treat digestive pathologies.Objective: We aimed to compare the gastroprotective effects of hydromethanolic leaves extracts of wild and cultivated grapes accessions native of Tunisia.Materials and methods: The phytochemical analysis of grapevine leaves extracts was performed. The gastroprotective activity was evaluated by ethanol-induced gastric-ulcer in rats pre-treated with increased doses of the extracts or with the standard omeprazole. Index of gastric secretions (volume, pH and gastric mucus production), stomach wall histology and biochemical parameters were estimated for assessment of anti-secretory and gastroprotective effects of the extracts. Results: Pre-treatment with grapevine leaves extracts decreased significantly gastric volume, gastric mucosal damage and increased significantly gastric juice pH compared with the negative control group. The extracts prevented ethanol-induced decrease of the activity of antioxidant enzymes while the levels of malondialdehyde and of reduced glutathione were decreased significantly. Moreover, the most marked effect was observed at low doses of wild ecotype 'Nefza-I' extracts. Conclusion: The leaves of Vitis species might be suitable as a functional food for therapeutic purpose and demonstrates gastroprotective action in gastric lesions model. Both accessions exhibited gastroprotective effects, but wild 'Nefza-I' ecotype was more effective than cultivar 'Marsaoui'.
Context: Vitis vinifera leaves are traditionally used in Tunisian folk medicine to treat digestive pathologies.Objective: We aimed to compare the gastroprotective effects of hydromethanolic leaves extracts of wild and cultivated grapes accessions native of Tunisia.Materials and methods: The phytochemical analysis of grapevine leaves extracts was performed. The gastroprotective activity was evaluated by ethanol-induced gastric-ulcer in rats pre-treated with increased doses of the extracts or with the standard omeprazole. Index of gastric secretions (volume, pH and gastric mucus production), stomach wall histology and biochemical parameters were estimated for assessment of anti-secretory and gastroprotective effects of the extracts. Results: Pre-treatment with grapevine leaves extracts decreased significantly gastric volume, gastric mucosal damage and increased significantly gastric juice pH compared with the negative control group. The extracts prevented ethanol-induced decrease of the activity of antioxidant enzymes while the levels of malondialdehyde and of reduced glutathione were decreased significantly. Moreover, the most marked effect was observed at low doses of wild ecotype 'Nefza-I' extracts. Conclusion: The leaves of Vitis species might be suitable as a functional food for therapeutic purpose and demonstrates gastroprotective action in gastric lesions model. Both accessions exhibited gastroprotective effects, but wild 'Nefza-I' ecotype was more effective than cultivar 'Marsaoui'.