Literature DB >> 31712780

Non-reversible tissue fixation retains extracellular vesicles for in situ imaging.

Mrinali P Gupta1, Sangeetha Tandalam1, Shariss Ostrager1, Alexander S Lever1, Angus R Fung1, David D Hurley1, Gemstonn B Alegre1, Jasmin E Espinal1, H Lawrence Remmel1,2, Sushmita Mukherjee1, Benjamin M Levine1, Russell P Robins3, Henrik Molina4, Brian D Dill4, Candia M Kenific5, Thomas Tuschl6, David Lyden5, Donald J D'Amico1, John T G Pena7.   

Abstract

Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and pathological associations. The inability to image EVs in fixed tissues has been a major limitation to understanding their role in healthy and diseased tissue microenvironments. Here, we show that crosslinking mammalian tissues with formaldehyde results in significant EV loss, which can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for visualization of EVs in a range of normal and cancer tissues.

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Year:  2019        PMID： 31712780      PMCID： PMC9344330          DOI： 10.1038/s41592-019-0623-4

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   47.990


  20 in total

1.  EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research.

Authors:  Jan Van Deun; Pieter Mestdagh; Patrizia Agostinis; Özden Akay; Sushma Anand; Jasper Anckaert; Zoraida Andreu Martinez; Tine Baetens; Els Beghein; Laurence Bertier; Geert Berx; Janneke Boere; Stephanie Boukouris; Michel Bremer; Dominik Buschmann; James B Byrd; Clara Casert; Lesley Cheng; Anna Cmoch; Delphine Daveloose; Eva De Smedt; Seyma Demirsoy; Victoria Depoorter; Bert Dhondt; Tom A P Driedonks; Aleksandra Dudek; Abdou Elsharawy; Ilaria Floris; Andrew D Foers; Kathrin Gärtner; Abhishek D Garg; Edward Geeurickx; Jan Gettemans; Farzaneh Ghazavi; Bernd Giebel; Tom Groot Kormelink; Grace Hancock; Hetty Helsmoortel; Andrew F Hill; Vincent Hyenne; Hina Kalra; David Kim; Joanna Kowal; Sandra Kraemer; Petra Leidinger; Carina Leonelli; Yaxuan Liang; Lien Lippens; Shu Liu; Alessandra Lo Cicero; Shaun Martin; Suresh Mathivanan; Prabhu Mathiyalagan; Támas Matusek; Gloria Milani; Marta Monguió-Tortajada; Liselot M Mus; Dillon C Muth; Andrea Németh; Esther N M Nolte-'t Hoen; Lorraine O'Driscoll; Roberta Palmulli; Michael W Pfaffl; Bjarke Primdal-Bengtson; Erminia Romano; Quentin Rousseau; Susmita Sahoo; Natalia Sampaio; Monisha Samuel; Benjamin Scicluna; Bieke Soen; Anneleen Steels; Johannes V Swinnen; Maarit Takatalo; Safia Thaminy; Clotilde Théry; Joeri Tulkens; Isabel Van Audenhove; Susanne van der Grein; Alan Van Goethem; Martijn J van Herwijnen; Guillaume Van Niel; Nadine Van Roy; Alexander R Van Vliet; Niels Vandamme; Suzanne Vanhauwaert; Glenn Vergauwen; Frederik Verweij; Annelynn Wallaert; Marca Wauben; Kenneth W Witwer; Marijke I Zonneveld; Olivier De Wever; Jo Vandesompele; An Hendrix
Journal:  Nat Methods       Date:  2017-02-28       Impact factor: 28.547

2.  miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications.

Authors:  Marco Ragusa; Cristina Barbagallo; Luisa Statello; Rosario Caltabiano; Andrea Russo; Lidia Puzzo; Teresio Avitabile; Antonio Longo; Mario D Toro; Davide Barbagallo; Hadi Valadi; Cinzia Di Pietro; Michele Purrello; Michele Reibaldi
Journal:  Cancer Biol Ther       Date:  2015-05-07       Impact factor: 4.742

3.  Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis.

Authors:  Rebecca A Dragovic; Christopher Gardiner; Alexandra S Brooks; Dionne S Tannetta; David J P Ferguson; Patrick Hole; Bob Carr; Christopher W G Redman; Adrian L Harris; Peter J Dobson; Paul Harrison; Ian L Sargent
Journal:  Nanomedicine       Date:  2011-05-04       Impact factor: 5.307

4.  Mouse 4T1 breast tumor model.

Authors:  B A Pulaski; S Ostrand-Rosenberg
Journal:  Curr Protoc Immunol       Date:  2001-05

Review 5.  Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles.

Authors:  Bence György; Tamás G Szabó; Mária Pásztói; Zsuzsanna Pál; Petra Misják; Borbála Aradi; Valéria László; Eva Pállinger; Erna Pap; Agnes Kittel; György Nagy; András Falus; Edit I Buzás
Journal:  Cell Mol Life Sci       Date:  2011-05-11       Impact factor: 9.261

6.  Extracellular vesicle-mediated transfer of functional RNA in the tumor microenvironment.

Authors:  Kirsten Ridder; Alexandra Sevko; Janina Heide; Maria Dams; Anne-Kathleen Rupp; Jadranka Macas; Julia Starmann; Marc Tjwa; Karl H Plate; Holger Sültmann; Peter Altevogt; Viktor Umansky; Stefan Momma
Journal:  Oncoimmunology       Date:  2015-03-19       Impact factor: 8.110

7.  In Vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior.

Authors:  Anoek Zomer; Carrie Maynard; Frederik Johannes Verweij; Alwin Kamermans; Ronny Schäfer; Evelyne Beerling; Raymond Michel Schiffelers; Elzo de Wit; Jordi Berenguer; Saskia Inge Johanna Ellenbroek; Thomas Wurdinger; Dirk Michiel Pegtel; Jacco van Rheenen
Journal:  Cell       Date:  2015-05-21       Impact factor: 41.582

8.  Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters.

Authors:  Charles P Lai; Edward Y Kim; Christian E Badr; Ralph Weissleder; Thorsten R Mempel; Bakhos A Tannous; Xandra O Breakefield
Journal:  Nat Commun       Date:  2015-05-13       Impact factor: 14.919

9.  Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.

Authors:  Johan Skog; Tom Würdinger; Sjoerd van Rijn; Dimphna H Meijer; Laura Gainche; Miguel Sena-Esteves; William T Curry; Bob S Carter; Anna M Krichevsky; Xandra O Breakefield
Journal:  Nat Cell Biol       Date:  2008-11-16       Impact factor: 28.824

10.  miRNA in situ hybridization in formaldehyde and EDC-fixed tissues.

Authors:  John T G Pena; Cherin Sohn-Lee; Sara H Rouhanifard; Janos Ludwig; Markus Hafner; Aleksandra Mihailovic; Cindy Lim; Daniel Holoch; Philipp Berninger; Mihaela Zavolan; Thomas Tuschl
Journal:  Nat Methods       Date:  2009-01-11       Impact factor: 28.547
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  8 in total

1.  K-means clustering of coherent Raman spectra from extracellular vesicles visualized by label-free multiphoton imaging.

Authors:  Yi Sun; Ethan W Chen; Jalen Thomas; Yuan Liu; Haohua Tu; Stephen A Boppart
Journal:  Opt Lett       Date:  2020-07-01       Impact factor: 3.776

Review 2.  Proteomics and Extracellular Vesicles as Novel Biomarker Sources in Peritoneal Dialysis in Children.

Authors:  Chiara Trincianti; Vincenzo Meleca; Edoardo La Porta; Maurizio Bruschi; Giovanni Candiano; Andrea Garbarino; Xhuliana Kajana; Alberto Preda; Francesca Lugani; Gian Marco Ghiggeri; Andrea Angeletti; Pasquale Esposito; Enrico Verrina
Journal:  Int J Mol Sci       Date:  2022-05-18       Impact factor: 6.208

Review 3.  Visualizing Extracellular Vesicles and Their Function in 3D Tumor Microenvironment Models.

Authors:  Evran E Ural; Victoria Toomajian; Ehsanul Hoque Apu; Mladen Veletic; Ilangko Balasingham; Nureddin Ashammakhi; Masamitsu Kanada; Christopher H Contag
Journal:  Int J Mol Sci       Date:  2021-04-30       Impact factor: 5.923

4.  Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy.

Authors:  Harris Sultan; Rithwick Rajagopal; Prabakar Kumar Rao; Kisha Deslee Piggott; Michael A Paley; Lynn Marisa Hassman; Albert S Li; Brigid Marshall; Rajendra Shridhar Apte
Journal:  Int J Ophthalmol       Date:  2022-01-18       Impact factor: 1.645

Review 5.  Potential roles of extracellular vesicles in the pathophysiology, diagnosis, and treatment of autoimmune diseases.

Authors:  Jing Tian; Giacomo Casella; Yuan Zhang; Abdolmohamad Rostami; Xing Li
Journal:  Int J Biol Sci       Date:  2020-01-01       Impact factor: 6.580

6.  A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles.

Authors:  Mikkel Ø Nørgård; Lasse B Steffensen; Didde R Hansen; Ernst-Martin Füchtbauer; Morten B Engelund; Henrik Dimke; Ditte C Andersen; Per Svenningsen
Journal:  Sci Rep       Date:  2022-01-11       Impact factor: 4.996

7.  In situ hybridization to detect DNA amplification in extracellular vesicles.

Authors:  Lucia Casadei; Patricia Sarchet; Fernanda Costas C de Faria; Federica Calore; Giovanni Nigita; Sayumi Tahara; Luciano Cascione; Martin Wabitsch; Francis J Hornicek; Valerie Grignol; Carlo M Croce; Raphael E Pollock
Journal:  J Extracell Vesicles       Date:  2022-09

Review 8.  Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing.

Authors:  Antonio Casado-Díaz; José Manuel Quesada-Gómez; Gabriel Dorado
Journal:  Front Bioeng Biotechnol       Date:  2020-03-03
  8 in total

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