Yoshihito Takahashi1,2, Takashi Fukuyama3, Nobue Futawatari4, Yoshinobu Ichiki5, Haruki Ohmiya6, Motohiro Chuman1,7, Tetsuro Yamashita6, Naoki Hiki7, Yusuke Kumamoto2, Hitoshi Yamazaki8, Hiroshi Nonoguchi9, Yatsushi Nishi1, Noritada Kobayashi10. 1. Department of Surgery, Kitasato University Medical Center, Kitamoto, Japan. 2. Department of General, Pediatric and Hepatobiliary-Pancreatic Surgery, Kitasato University, Sagamihara, Japan. 3. Department of Biomedical Research, Kitasato University Medical Center, Kitamoto, Japan fukuyam@insti.kitasato-u.ac.jp. 4. Department of General and Gastroenterological Surgery, Department of Surgery (Ohashi), Toho University, Tokyo, Japan. 5. Department of General Thoracic Surgery, National Hospital Organization Saitama Hospital, Wako, Japan. 6. Department of Biological Chemistry and Food Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan. 7. Department of Upper Gastrointestinal Surgery, Kitasato University School of Medicine, Sagamihara, Japan. 8. Department of Pathology, Kitasato University Medical Center, Kitamoto, Japan. 9. Department of Internal Medicine, Kitasato University Medical Center, Kitamoto, Japan. 10. Department of Biomedical Research, Kitasato University Medical Center, Kitamoto, Japan.
Abstract
BACKGROUND/AIM: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a known cancer/testis antigen. Our group has previously shown KK-LC-1 gene expression in gastric cancer. However, could not be detected the KK-LC-1 protein due to the lack of an appropriate antibody. Here, we assessed our original monoclonal antibody (Kmab34B3) and, using it, assessed the expression of KK-LC-1 in gastric cancer. PATIENTS AND METHODS: We evaluated an original monoclonal antibody against KK-LC-1 (Kmab34B3), and used this antibody to compare KK-LC-1 protein expression in tumour and non-tumour stomach cells from gastric cancer patients. RESULTS: Kmab34B3 stained testicular germ cells, and tumour cells in nine out of 11 (82%) specimens. In non-tumorous areas, Kmab34B3 stained 13 out of 29 (45%) pyloric gland specimens. Furthermore, Kmab34B3 also stained intestinal metaplasia positive and negative areas. CONCLUSION: Kmab34B3 was able to detect KK-LC-1 protein within tumour cells and the pyloric gland where the gene has been shown to be expressed. Therefore, it might be an attractive tool for detecting KK-LC-1 expression in precancerous and cancerous stomach cells. Copyright
BACKGROUND/AIM: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a known cancer/testis antigen. Our group has previously shown KK-LC-1 gene expression in gastric cancer. However, could not be detected the KK-LC-1 protein due to the lack of an appropriate antibody. Here, we assessed our original monoclonal antibody (Kmab34B3) and, using it, assessed the expression of KK-LC-1 in gastric cancer. PATIENTS AND METHODS: We evaluated an original monoclonal antibody against KK-LC-1 (Kmab34B3), and used this antibody to compare KK-LC-1 protein expression in tumour and non-tumour stomach cells from gastric cancerpatients. RESULTS: Kmab34B3 stained testicular germ cells, and tumour cells in nine out of 11 (82%) specimens. In non-tumorous areas, Kmab34B3 stained 13 out of 29 (45%) pyloric gland specimens. Furthermore, Kmab34B3 also stained intestinal metaplasia positive and negative areas. CONCLUSION: Kmab34B3 was able to detect KK-LC-1 protein within tumour cells and the pyloric gland where the gene has been shown to be expressed. Therefore, it might be an attractive tool for detecting KK-LC-1 expression in precancerous and cancerous stomach cells. Copyright