| Literature DB >> 31701457 |
Lucy Jade Newbury1, Alexa Wonnacott1, Kate Simpson1, Timothy Bowen1, Donald Fraser2.
Abstract
Urinary microRNAs show promise as noninvasive biomarkers in renal disease. Here, we describe a detailed protocol for the column-based extraction and quantification of miRNAs by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) from urine samples.Entities:
Keywords: Biomarker; Chronic kidney disease; Diabetic nephropathy; MicroRNAs; RT-qPCR; Urine
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Year: 2020 PMID： 31701457 DOI： 10.1007/978-1-4939-9841-8_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745