Can Feng1,2, Qi Chen3, Min Fan2, Jun Guo1, Yu Liu4, Tao Ji5, Jiaqi Zhu1, Xianxian Zhao1. 1. Department of Cardiology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. 2. Department of Cardiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. 3. Department of Cardiology, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China. 4. Department of Cardiology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing 210008, China. 5. Department of Neurosurgery, Tenth Affiliated Hospital, Tongji University, Shanghai 200072, China.
Abstract
BACKGROUND: The interaction between platelets and macrophages plays an important role in the development and progression of atherosclerosis (AS). This study aimed to investigate the role of platelet microparticles (PMPs) in the development of foam cells. METHODS: PMPs are generated by activating platelets with thrombin and separated by ultracentrifugation. The macrophages were treated with PMPs, the phagocytosis of oxidized low-density lipoprotein (Ox-LDL) and formation of foam cells were evaluated by flow cytometry and confocal microscopy, respectively, and the inflammatory factors cytokines in the supernatant were detected by ELISA. RESULTS: PMPs significantly increase the phagocytosis of Ox-LDL and elevated foam cell formation of macrophages. IL-1β content in the supernatant of macrophages peaked around 2-4 h and declined to normal level after 6-8 h; IL-6 content peaked at 4 h and then decreased to normal level. TNF-α content peaked at 2-4 h. CONCLUSIONS: The microparticles from activated platelets can increase the phagocytosis of Ox-LDL and the production of inflammatory cytokines by macrophages, which is related to the development of AS. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: The interaction between platelets and macrophages plays an important role in the development and progression of atherosclerosis (AS). This study aimed to investigate the role of platelet microparticles (PMPs) in the development of foam cells. METHODS: PMPs are generated by activating platelets with thrombin and separated by ultracentrifugation. The macrophages were treated with PMPs, the phagocytosis of oxidized low-density lipoprotein (Ox-LDL) and formation of foam cells were evaluated by flow cytometry and confocal microscopy, respectively, and the inflammatory factors cytokines in the supernatant were detected by ELISA. RESULTS: PMPs significantly increase the phagocytosis of Ox-LDL and elevated foam cell formation of macrophages. IL-1β content in the supernatant of macrophages peaked around 2-4 h and declined to normal level after 6-8 h; IL-6 content peaked at 4 h and then decreased to normal level. TNF-α content peaked at 2-4 h. CONCLUSIONS: The microparticles from activated platelets can increase the phagocytosis of Ox-LDL and the production of inflammatory cytokines by macrophages, which is related to the development of AS. 2019 Annals of Translational Medicine. All rights reserved.
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