Haoming Zhou1,2, Feng Zhan1,2,3, Hui Zhang1,2, Jian Gu1,2, Xiaoxin Mu1,2, Ji Gao1,2, Jianhua Rao1,2, Guwei Ji1,2, Xuhao Ni1,2, Ling Lu1,2, Yongxiang Xia1,2. 1. Translational Medicine Research Center of Affiliated Jiangning Hospital, Liver Transplantation Center of First Affiliated Hospital, and Collaborative Innovation Center for Cancer Medicine, Nanjing Medical University, Nanjing 210029, China. 2. Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, Nanjing 210029, China. 3. Department of Hepatobiliary and Laparoscopic Surgery, the Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
Abstract
BACKGROUND: Regulatory B cells (Bregs) play an essential role in inflammation and transplant tolerance. Several studies have reported a decreased number of Bregs in renal transplant patients with graft rejection. However, little is known about their role in the liver alloresponse. METHODS: To investigate whether the circulating Bregs have been associated with acute allograft rejection (AR) in liver transplantation patients, 19 patients receiving liver allografts from donation after cardiac death (DCD) donors were retrospectively studied. RESULTS: The postoperative proportions of circulating CD19+CD24hiCD38hi transitional Bregs (tBregs) and CD19+CD24hiCD27+ memory Bregs (mBregs) in patients diagnosed with AR (AR group) and other patients with stable allograft liver function (SF group) were evaluated using flow cytometry (FCM) analysis. Results showed that while no significant changes were found regarding both the tBreg and mBreg, proportions across all time points in the SF group, the AR group showed significantly decreased proportions of mBregs. All of the five AR patients responded fine to the treatments, and the proportions of mBregs increased significantly after anti-rejection therapies. In addition, AR was suspected in four recipients, but gradually they were diagnosed with hemolytic or obstructive jaundice and showed no decrease in the proportion of mBregs. CONCLUSIONS: For the first time, our results suggested the potential role of a decreased proportion of circulating mBregs in predicting AR in patients with post liver transplantation. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: Regulatory B cells (Bregs) play an essential role in inflammation and transplant tolerance. Several studies have reported a decreased number of Bregs in renal transplant patients with graft rejection. However, little is known about their role in the liver alloresponse. METHODS: To investigate whether the circulating Bregs have been associated with acute allograft rejection (AR) in liver transplantation patients, 19 patients receiving liver allografts from donation after cardiac death (DCD) donors were retrospectively studied. RESULTS: The postoperative proportions of circulating CD19+CD24hiCD38hi transitional Bregs (tBregs) and CD19+CD24hiCD27+ memory Bregs (mBregs) in patients diagnosed with AR (AR group) and other patients with stable allograft liver function (SF group) were evaluated using flow cytometry (FCM) analysis. Results showed that while no significant changes were found regarding both the tBreg and mBreg, proportions across all time points in the SF group, the AR group showed significantly decreased proportions of mBregs. All of the five AR patients responded fine to the treatments, and the proportions of mBregs increased significantly after anti-rejection therapies. In addition, AR was suspected in four recipients, but gradually they were diagnosed with hemolytic or obstructive jaundice and showed no decrease in the proportion of mBregs. CONCLUSIONS: For the first time, our results suggested the potential role of a decreased proportion of circulating mBregs in predicting AR in patients with post liver transplantation. 2019 Annals of Translational Medicine. All rights reserved.
Entities:
Keywords:
Liver transplantation; acute allograft rejection; donation after cardiac death (DCD); liver function; regulatory B cells
Authors: Girdhari Lal; Yumi Nakayama; Apoorva Sethi; Amit K Singh; Bryna E Burrell; Neeraja Kulkarni; C Colin Brinkman; Daiki Iwami; Tianshu Zhang; Jonathan S Bromberg Journal: Transplantation Date: 2015-09 Impact factor: 4.939
Authors: Giacomo Germani; Kryssia Rodriguez-Castro; Francesco Paolo Russo; Marco Senzolo; Alberto Zanetto; Alberto Ferrarese; Patrizia Burra Journal: World J Gastroenterol Date: 2015-01-28 Impact factor: 5.742
Authors: Siddharth Sood; Craig Haifer; Lijia Yu; Julie Pavlovic; Leonid Churilov; Paul J Gow; Robert M Jones; Peter W Angus; Kumar Visvanathan; Adam G Testro Journal: Liver Transpl Date: 2017-04 Impact factor: 5.799