Yuqin Zhang1,2,3, Lin Zheng4, Xuejun Lao5, Mingbo Wen5, Zhipeng Qian1, Xin Liu1, Hui Tang6, Fei Gao1,7,8. 1. Laboratory of Digestive Disease and Oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. 2. Department of Oncology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. 3. Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. 4. Department of Pathology, Nanfang Hospital and School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. 5. Department of Gastrointestinal Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. 6. Central Laboratory, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. 7. Department of Gastroenterology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China. 8. Department of Physiology and Biomedical Engineering and Gastroenterology Research Unit, Enteric Neuroscience Program, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA.
Abstract
BACKGROUND: Long noncoding RNAs (lncRNAs) play important roles in the development and pathophysiology of colorectal cancer (CRC). Our previous study showed that Hes1 was involved in the self-renewal and tumorigenicity of stem-like cancer cells in CRC. METHODS: ArrayStar Human LncRNA/mRNA Expression Microarray Version 3.0 was used to detect lncRNA expression in CRC tissues compared with their matched non-tumoral tissues. RNA-binding protein immunoprecipitation and sequencing (RIP-seq) assay was used to detect lncRNAs binding to Hes1. Real-time qPCR was used to detect expression of specific lncRNAs in CRC tissues. RESULTS: We found significantly up-regulated as well as down-regulated lncRNAs in CRC tissues compared with their matched non-tumoral tissues. We also screened a number of lncRNAs interacting with Hes1 in CRC cells. Interestingly, we found several lncRNAs binding to Hes1 (such as, GNAS-AS1, RP11-89K10.1, and RP11-465L10.10) were up-regulated in CRC tissues showed by the tissue microarray. Next, we confirmed that Hes1 directly interacted with these lncRNAs using RIP-qPCR and RNA pulldown assay. Finally, we verified the expression of these lncRNAs in 32 CRC samples as well as the adjacent non-tumoral tissues using real-time qPCR. CONCLUSIONS: Based on these, we speculate that Hes1 interacts with one or more lncRNAs which contribute to the development and progression of CRC. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: Long noncoding RNAs (lncRNAs) play important roles in the development and pathophysiology of colorectal cancer (CRC). Our previous study showed that Hes1 was involved in the self-renewal and tumorigenicity of stem-like cancer cells in CRC. METHODS: ArrayStar Human LncRNA/mRNA Expression Microarray Version 3.0 was used to detect lncRNA expression in CRC tissues compared with their matched non-tumoral tissues. RNA-binding protein immunoprecipitation and sequencing (RIP-seq) assay was used to detect lncRNAs binding to Hes1. Real-time qPCR was used to detect expression of specific lncRNAs in CRC tissues. RESULTS: We found significantly up-regulated as well as down-regulated lncRNAs in CRC tissues compared with their matched non-tumoral tissues. We also screened a number of lncRNAs interacting with Hes1 in CRC cells. Interestingly, we found several lncRNAs binding to Hes1 (such as, GNAS-AS1, RP11-89K10.1, and RP11-465L10.10) were up-regulated in CRC tissues showed by the tissue microarray. Next, we confirmed that Hes1 directly interacted with these lncRNAs using RIP-qPCR and RNA pulldown assay. Finally, we verified the expression of these lncRNAs in 32 CRC samples as well as the adjacent non-tumoral tissues using real-time qPCR. CONCLUSIONS: Based on these, we speculate that Hes1 interacts with one or more lncRNAs which contribute to the development and progression of CRC. 2019 Annals of Translational Medicine. All rights reserved.
Entities:
Keywords:
Hes1; Long non-coding RNAs (lncRNAs); colorectal cancer (CRC)
Authors: V Mouraviev; B Lee; V Patel; D Albala; T E B Johansen; A Partin; A Ross; R J Perera Journal: Prostate Cancer Prostatic Dis Date: 2015-10-27 Impact factor: 5.554