Zhanqi Li1, Shaoxiong Zhao1, Hui Wang1, Binbin Zhang2, Peibo Zhang2. 1. Department of Urology, Shaanxi Nuclear Industry 215 Hospital, Xianyang, P. R. China. 2. Department of Urology, Affiliated hospital of Yan'an university, Yan'an, P. R. China.
Abstract
BACKGROUND: Prostate cancer (PCa) is a heavy health-threat for human worldwide. Recent studies revealed that microRNAs (miRNAs) are associated with the progression of human cancers, including PCa. However, no study has been performed to investigate the role of microNA-4286 (miR-4286) on PCa. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted to analyze the expression level of miR-4286 in PCa cells. Connection of miR-4286 and spalt like transcription factor 1 (SALL1) was analyzed with bioinformatic analysis tool, dual-luciferase activity reporter assay, and western blot. Effects of miR-4286 and SALL1 on PCa cell behaviors were examined in vitro. RESULTS: We showed miR-4286 expression was significantly increased in PCa cells compared with normal cell line. Knockdown of miR-4286 could inhibit PCa cell proliferation but promote cell apoptosis via targeting SALL1. CONCLUSIONS: Our study suggested that miR-4286 overexpression represents a tumor promoter role in PCa. This article is protected by copyright. All rights reserved.
BACKGROUND:Prostate cancer (PCa) is a heavy health-threat for human worldwide. Recent studies revealed that microRNAs (miRNAs) are associated with the progression of humancancers, including PCa. However, no study has been performed to investigate the role of microNA-4286 (miR-4286) on PCa. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted to analyze the expression level of miR-4286 in PCa cells. Connection of miR-4286 and spalt like transcription factor 1 (SALL1) was analyzed with bioinformatic analysis tool, dual-luciferase activity reporter assay, and western blot. Effects of miR-4286 and SALL1 on PCa cell behaviors were examined in vitro. RESULTS: We showed miR-4286 expression was significantly increased in PCa cells compared with normal cell line. Knockdown of miR-4286 could inhibit PCa cell proliferation but promote cell apoptosis via targeting SALL1. CONCLUSIONS: Our study suggested that miR-4286 overexpression represents a tumor promoter role in PCa. This article is protected by copyright. All rights reserved.