A Z Mahmoud1,2, H A Ibrahim2, M R El-Sawi2, M N Habza2. 1. Urology and Nephrology Center, Mansoura University, Mansoura, Egypt. 2. Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Abstract
Purpose: The present study was undertaken to evaluate the protective and therapeutic effects of silymarin and mesenchymal stem cells (MSCs) to ameliorate the damage caused by gamma radiation.Materials and methods: MSCs were given by intravenous injection to male rats (1.4 × 107 cells), 1 day next to gamma radiation (4Gy). While, silymarin was administered orally at a dose of 70 mg/kg b. wt., 3 days before irradiation and continued for 21 days post irradiation. Results: After 1 and 3 weeks post-irradiation, the results revealed a significant decline in red blood corpuscles (RBCs), white blood corpuscles (WBCs) and platelets count with rising in serum lipid profile [total lipids (TL), total glycerides (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) levels] and total bilirubin; while significant decreases in serum total protein and high density lipoprotein-cholesterol (HDL-C) levels were observed. In irradiated animals receiving double treatment with MSCs and silymarin; amelioration of the changes observed in hematological and biochemical parameters when comparing with the irradiated group.Conclusions: Treatment with a radio-protector (such as silymarin) in addition to MSCs transplantation was recommended to protect against gamma radiation injury.
Purpose: The present study was undertaken to evaluate the protective and therapeutic effects of silymarin and mesenchymal stem cells (MSCs) to ameliorate the damage caused by gamma radiation.Materials and methods: MSCs were given by intravenous injection to male rats (1.4 × 107 cells), 1 day next to gamma radiation (4Gy). While, silymarin was administered orally at a dose of 70 mg/kg b. wt., 3 days before irradiation and continued for 21 days post irradiation. Results: After 1 and 3 weeks post-irradiation, the results revealed a significant decline in red blood corpuscles (RBCs), white blood corpuscles (WBCs) and platelets count with rising in serum lipid profile [total lipids (TL), total glycerides (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) levels] and total bilirubin; while significant decreases in serum total protein and high density lipoprotein-cholesterol (HDL-C) levels were observed. In irradiated animals receiving double treatment with MSCs and silymarin; amelioration of the changes observed in hematological and biochemical parameters when comparing with the irradiated group.Conclusions: Treatment with a radio-protector (such as silymarin) in addition to MSCs transplantation was recommended to protect against gamma radiation injury.