| Literature DB >> 31691450 |
Rafael Calero-Bernal1, Mónica Santín2, Jenny G Maloney2, Manuel Martín-Pérez3, Miguel A Habela3, José L Fernández-García3, Ana Figueiredo4, Fernando Nájera5, María J Palacios5, Marta Mateo6, Ana Balseiro7, Marta Barral8, José F Lima-Barberoi9,10, Pamela C Köster11, David Carmena11.
Abstract
The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.Entities:
Keywords: Next generation sequencing; Sanger; wildlife; zoonotic transmission
Mesh：
Year: 2019 PMID： 31691450 DOI： 10.1111/jeu.12772
Source DB: PubMed Journal: J Eukaryot Microbiol ISSN： 1066-5234 Impact factor: 3.346