Literature DB >> 31687748

Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples.

Tomohiro Haruta1, Keiko Hasumi1, Yuta Ikeda1, Yuji Konyuba1, Tomohisa Fukuda1, Hideo Nishioka1.   

Abstract

In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.
© The Author(s) 2019. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Keywords:  Au NPs; LSPR; SiN film; correlative microscopy; imaging tag

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Year:  2019        PMID： 31687748     DOI： 10.1093/jmicro/dfz031

Source DB:  PubMed          Journal:  Microscopy (Oxf)        ISSN： 2050-5698            Impact factor:   1.571


  2 in total

1.  Correlative light and electron microscopy of poly(ʟ-lactic acid) spherulites for fast morphological measurements using a convolutional neural network.

Authors:  Yuji Konyuba; Hironori Marubayashi; Tomohiro Haruta; Hiroshi Jinnai
Journal:  Microscopy (Oxf)       Date:  2022-04-01       Impact factor: 1.571

Review 2.  Nanoscopy for endosomal escape quantification.

Authors:  Teodora Andrian; Roger Riera; Silvia Pujals; Lorenzo Albertazzi
Journal:  Nanoscale Adv       Date:  2020-10-27
  2 in total

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