Libing Yang1,2,3, Daniel G Dunlap1, Shulin Qin1,2, Adam Fitch2, Kelvin Li2, Carl D Koch1,2, Mehdi Nouraie1, Rebecca DeSensi1, Ken S Ho4, Jeremy J Martinson5, Barbara Methé1,2, Alison Morris1,2,6. 1. Division of Pulmonary, Allergy and Critical Care Medicine and. 2. Center for Medicine and the Microbiome, Department of Medicine. 3. School of Medicine, Tsinghua University, Beijing, China; and. 4. Division of Infectious Disease, Department of Medicine, and. 5. Infectious Diseases and Microbiology, University of Pittsburgh School of Public Health, Pittsburgh, Pennsylvania. 6. Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Abstract
Rationale: Mechanisms of HIV-associated chronic obstructive pulmonary disease (COPD) are poorly understood. The oral microbiome shapes the lung microbiome, and gut dysbiosis can affect lung diseases; however, relationships of the oral and gut microbiome to COPD in HIV have not been explored. Objectives: To examine alterations in the oral and gut microbiome associated with pulmonary disease in persons with HIV (PWH). Methods: Seventy-five PWH and 93 HIV-uninfected men from the MACS (Multicenter AIDS Cohort Study) performed pulmonary function testing. Sequencing of bacterial 16S ribosomal RNA in saliva and stool was performed. We used nonmetric multidimensional scaling, permutational multivariate ANOVA, and linear discriminant analysis to analyze communities by HIV and lung function.Measurements and Main Results: Oral microbiome composition differed by HIV and smoking status. Alterations of oral microbial communities were observed in PWH with abnormal lung function with increases in relative abundance of Veillonella, Streptococcus, and Lactobacillus. There were no significant associations between the oral microbiome and lung function in HIV-uninfected individuals. No associations with HIV status or lung function were seen with the gut microbiome.Conclusions: Alterations of oral microbiota in PWH were related to impaired pulmonary function and to systemic inflammation. These results suggest that the oral microbiome may serve as a biomarker of lung function in HIV and that its disruption may contribute to COPD pathogenesis.
Rationale: Mechanisms of HIV-associated chronic obstructive pulmonary disease (COPD) are poorly understood. The oral microbiome shapes the lung microbiome, and gut dysbiosis can affect lung diseases; however, relationships of the oral and gut microbiome to COPD in HIV have not been explored. Objectives: To examine alterations in the oral and gut microbiome associated with pulmonary disease in persons with HIV (PWH). Methods: Seventy-five PWH and 93 HIV-uninfectedmen from the MACS (Multicenter AIDS Cohort Study) performed pulmonary function testing. Sequencing of bacterial 16S ribosomal RNA in saliva and stool was performed. We used nonmetric multidimensional scaling, permutational multivariate ANOVA, and linear discriminant analysis to analyze communities by HIV and lung function.Measurements and Main Results: Oral microbiome composition differed by HIV and smoking status. Alterations of oral microbial communities were observed in PWH with abnormal lung function with increases in relative abundance of Veillonella, Streptococcus, and Lactobacillus. There were no significant associations between the oral microbiome and lung function in HIV-uninfected individuals. No associations with HIV status or lung function were seen with the gut microbiome.Conclusions: Alterations of oral microbiota in PWH were related to impaired pulmonary function and to systemic inflammation. These results suggest that the oral microbiome may serve as a biomarker of lung function in HIV and that its disruption may contribute to COPD pathogenesis.
Authors: Jose Luis Sandoval-Gutierrez; Juan Rodriguez-Silverio; Rosa Maria Rivera-Rosales; Edgar Sevilla-Reyes; Francisco Javier Flores-Murrieta; Jorge Rojas-Serrano; Gustavo Reyes-Teran Journal: Arch Med Res Date: 2015-04-17 Impact factor: 2.235
Authors: Emily S Charlson; Jun Chen; Rebecca Custers-Allen; Kyle Bittinger; Hongzhe Li; Rohini Sinha; Jennifer Hwang; Frederic D Bushman; Ronald G Collman Journal: PLoS One Date: 2010-12-20 Impact factor: 3.240
Authors: L M Rocha-Ramírez; R A Pérez-Solano; S L Castañón-Alonso; S S Moreno Guerrero; A Ramírez Pacheco; M García Garibay; C Eslava Journal: J Immunol Res Date: 2017-07-05 Impact factor: 4.818
Authors: Jin J Zhou; Jing Zhai; Hua Zhou; Yin Chen; Stefano Guerra; Ian Robey; George M Weinstock; Erica Weinstock; Qunfeng Dong; Kenneth S Knox; Homer L Twigg Journal: Am J Respir Crit Care Med Date: 2020-12-15 Impact factor: 21.405
Authors: Sylvia A D Rofael; James Brown; Elisha Pickett; Margaret Johnson; John R Hurst; David Spratt; Marc Lipman; Timothy D McHugh Journal: EClinicalMedicine Date: 2020-06-27
Authors: Fariha N Ananya; Md Ripon Ahammed; Michael M Fahem; Sunam Kafle; Mahima Viswanathan; Darshi Desai; Radhika Akku; Faryal Khan; Tabata E Hernandez; Supreet K Bala; Shivam Gulati; Natalia Martin; George D Yatzkan; Javier Pérez-Fernández Journal: Cureus Date: 2021-11-07
Authors: Sudha Bhadriraju; Douglas W Fadrosh; Susan V Lynch; John Z Metcalfe; Meera K Shenoy; Din L Lin; Kole V Lynch; Kathryn McCauley; Rashida A Ferrand; Edith D Majonga; Grace McHugh; Laurence Huang Journal: Sci Rep Date: 2020-09-30 Impact factor: 4.996