Brooke L Deatherage Kaiser1, Jon M Jacobs2, Athena A Schepmoes2, Heather M Brewer3, Bobbie-Jo M Webb-Robertson2, Sandra Valtier4, Vikhyat S Bebarta5, Joshua N Adkins2. 1. Chemical and Biological Signature Sciences Group, Pacific Northwest National Laboratory, Richland, WA, USA. 2. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. 3. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA. 4. Science and Technology, 59th Medical Wing, JBSA-Lackland AFB, San Antonio, TX, USA. 5. University of Colorado School of Medicine, Aurora, CO, USA. Vikhyat.bebarta@ucdenver.edu.
Abstract
INTRODUCTION: Non-medical use and abuse of prescription opioids is a growing problem in both the civilian and military communities, with minimal technologies for detecting hydrocodone use. This study explored the proteomic changes that occur in the oral fluid and blood plasma following controlled hydrocodone administration in 20 subjects. METHODS: The global proteomic profile was determined for samples taken at four time points per subject: pre-exposure and 4, 6, or 168 hours post-exposure. The oral fluid samples analyzed herein provided greater differentiation between baseline and response time points than was observed with blood plasma, at least partially due to significant person-to-person relative variability in the plasma proteome. RESULTS: A total of 399 proteins were identified from oral fluid samples, and the abundance of 118 of those proteins was determined to be significantly different upon metabolism of hydrocodone (4 and 6 hour time points) as compared to baseline levels in the oral fluid (pre-dose and 168 hours). CONCLUSIONS: We present an assessment of the oral fluid and plasma proteome following hydrocodone administration, which demonstrates the potential of oral fluid as a noninvasive sample that may reveal features of hydrocodone in opioid use, and with additional study, may be useful for other opioids and in settings of misuse.
INTRODUCTION: Non-medical use and abuse of prescription opioids is a growing problem in both the civilian and military communities, with minimal technologies for detecting hydrocodone use. This study explored the proteomic changes that occur in the oral fluid and blood plasma following controlled hydrocodone administration in 20 subjects. METHODS: The global proteomic profile was determined for samples taken at four time points per subject: pre-exposure and 4, 6, or 168 hours post-exposure. The oral fluid samples analyzed herein provided greater differentiation between baseline and response time points than was observed with blood plasma, at least partially due to significant person-to-person relative variability in the plasma proteome. RESULTS: A total of 399 proteins were identified from oral fluid samples, and the abundance of 118 of those proteins was determined to be significantly different upon metabolism of hydrocodone (4 and 6 hour time points) as compared to baseline levels in the oral fluid (pre-dose and 168 hours). CONCLUSIONS: We present an assessment of the oral fluid and plasma proteome following hydrocodone administration, which demonstrates the potential of oral fluid as a noninvasive sample that may reveal features of hydrocodone in opioid use, and with additional study, may be useful for other opioids and in settings of misuse.
Authors: Ryan T Kelly; Jason S Page; Quanzhou Luo; Ronald J Moore; Daniel J Orton; Keqi Tang; Richard D Smith Journal: Anal Chem Date: 2006-11-15 Impact factor: 6.986
Authors: Anoop M Mayampurath; Navdeep Jaitly; Samuel O Purvine; Matthew E Monroe; Kenneth J Auberry; Joshua N Adkins; Richard D Smith Journal: Bioinformatics Date: 2008-02-26 Impact factor: 6.937
Authors: Viktor Granholm; Sangtae Kim; José C F Navarro; Erik Sjölund; Richard D Smith; Lukas Käll Journal: J Proteome Res Date: 2013-12-23 Impact factor: 4.466
Authors: Simone Nicolardi; Bogdan Bogdanov; André M Deelder; Magnus Palmblad; Yuri E M van der Burgt Journal: Int J Mol Sci Date: 2015-11-13 Impact factor: 5.923