Jin Li1, HongBin Fang2, Fang Jiang3, Yang Ning1. 1. Department of Medicine, Fuyang Hospital, 100 Qinghe Road, Fuyang, Anhui, 236037, PR China. 2. Department of Biostatistics, Bioinformatics & Biomathematics, Georgetown University Medical Center, 4000 Reservoir Road, NW, Washington DC 20057, USA. 3. Cancer Center, University of Maryland Baltimore, N9E17 22 S. Greene Street, Baltimore, 21201 MD, USA.
Abstract
Aim: We externally validate plasma miRNAs biomarkers for lung cancer in a large and retrospective sample set collected from a geographically distant population. Methods: Plasma samples are tested blindly to the clinical annotations by using PCR for quantitation of the four miRNAs in cohort 1 consisting of 232 lung cancer cases and 243 controls and cohort 2 comprising 239 cases and 246 controls. Results: Combined use of the four plasma miRNAs has 91% sensitivity and 95% specificity for diagnosis of lung cancer, and 85% sensitivity for early-stage lung cancer, while maintaining a specificity of 95%. Conclusion: The diagnostic values of the biomarkers are reproducibly confirmed in the independent and large sample sets, providing an assay for lung cancer detection.
Aim: We externally validate plasma miRNAs biomarkers for lung cancer in a large and retrospective sample set collected from a geographically distant population. Methods: Plasma samples are tested blindly to the clinical annotations by using PCR for quantitation of the four miRNAs in cohort 1 consisting of 232 lung cancer cases and 243 controls and cohort 2 comprising 239 cases and 246 controls. Results: Combined use of the four plasma miRNAs has 91% sensitivity and 95% specificity for diagnosis of lung cancer, and 85% sensitivity for early-stage lung cancer, while maintaining a specificity of 95%. Conclusion: The diagnostic values of the biomarkers are reproducibly confirmed in the independent and large sample sets, providing an assay for lung cancer detection.
Authors: Martina Faraldi; Laura Gerosa; Marta Gomarasca; Veronica Sansoni; Silvia Perego; Ewa Ziemann; Giuseppe Banfi; Giovanni Lombardi Journal: Diagnostics (Basel) Date: 2021-04-30
Authors: Jody Vykoukal; Johannes F Fahrmann; Nikul Patel; Masayoshi Shimizu; Edwin J Ostrin; Jennifer B Dennison; Cristina Ivan; Gary E Goodman; Mark D Thornquist; Matt J Barnett; Ziding Feng; George A Calin; Samir M Hanash Journal: Cancers (Basel) Date: 2022-08-30 Impact factor: 6.575