Pinelopi I Artemaki1, Aimilia D Sklirou2, Christos K Kontos1, Aikaterini-Anna Liosi1, Despoina D Gianniou2, Iordanis N Papadopoulos3, Ioannis P Trougakos4, Andreas Scorilas5. 1. Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens GR-15701, Greece. 2. Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens GR-15784, Greece. 3. Fourth Surgery Department, University General Hospital "Attikon", Athens GR-12462, Greece. 4. Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens GR-15784, Greece. Electronic address: itrougakos@biol.uoa.gr. 5. Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens GR-15701, Greece. Electronic address: ascorilas@biol.uoa.gr.
Abstract
OBJECTIVES: Clusterin (CLU) is a multifunctional intra-/extra-cellular molecular chaperone with indications of serving as a promising prognostic biomarker for colorectal cancer (CRC). Several studies have examined the potential prognostic value of the CLU protein in CRC; however, our research follows an alternative approach, focusing on the CLU mRNA expression. DESIGN AND METHODS: Total RNA from 172 cancerous tissue specimens and 39 paired non-cancerous ones was isolated and 2 μg of this were subjected to reverse transcription with an oligo-dT primer. The single stranded DNA, which was synthesized, was amplified with an in-house developed highly sensitive and precise qPCR method, using specific pair of primers for the CLU molecule. Finally, an extensive biostatistical analysis took place for the assessment of the results. RESULTS: Patients with tumors expressing high CLU mRNA levels had a higher probability for poor outcome (relapse and death), comparing to those with CLU mRNA-negative tumors. This association between CLU mRNA expression status and both disease-free survival (DFS) and overall survival (OS) is evident in Cox regression analysis and is also depicted in the Kaplan-Meier survival curves. Consistently, the aforementioned associations and the CLU mRNA expression levels are significantly enhanced as CRC tumors progress from TNM stage I to IV, further supporting the functional implication of CLU in tumorigenesis. CONCLUSIONS: High CLU mRNA levels in CRC tumors can act as a new adverse prognostic biomarker of DFS and OS for CRC, independent of clinicopathological and biological features of the patient.
OBJECTIVES:Clusterin (CLU) is a multifunctional intra-/extra-cellular molecular chaperone with indications of serving as a promising prognostic biomarker for colorectal cancer (CRC). Several studies have examined the potential prognostic value of the CLU protein in CRC; however, our research follows an alternative approach, focusing on the CLU mRNA expression. DESIGN AND METHODS: Total RNA from 172 cancerous tissue specimens and 39 paired non-cancerous ones was isolated and 2 μg of this were subjected to reverse transcription with an oligo-dT primer. The single stranded DNA, which was synthesized, was amplified with an in-house developed highly sensitive and precise qPCR method, using specific pair of primers for the CLU molecule. Finally, an extensive biostatistical analysis took place for the assessment of the results. RESULTS:Patients with tumors expressing high CLU mRNA levels had a higher probability for poor outcome (relapse and death), comparing to those with CLU mRNA-negative tumors. This association between CLU mRNA expression status and both disease-free survival (DFS) and overall survival (OS) is evident in Cox regression analysis and is also depicted in the Kaplan-Meier survival curves. Consistently, the aforementioned associations and the CLU mRNA expression levels are significantly enhanced as CRCtumors progress from TNM stage I to IV, further supporting the functional implication of CLU in tumorigenesis. CONCLUSIONS: High CLU mRNA levels in CRCtumors can act as a new adverse prognostic biomarker of DFS and OS for CRC, independent of clinicopathological and biological features of the patient.
Authors: Paraskevi Karousi; Pinelopi I Artemaki; Christina D Sotiropoulou; Spyridon Christodoulou; Andreas Scorilas; Christos K Kontos Journal: Int J Mol Sci Date: 2020-11-23 Impact factor: 5.923
Authors: Aimilia D Sklirou; Despoina D Gianniou; Paraskevi Karousi; Christina Cheimonidi; Georgia Papachristopoulou; Christos K Kontos; Andreas Scorilas; Ioannis P Trougakos Journal: Int J Mol Sci Date: 2022-08-28 Impact factor: 6.208
Authors: Xuezhi Du; Han Qi; Wenbin Ji; Peiyuan Li; Run Hua; Wenliang Hu; Feng Qi Journal: Comput Math Methods Med Date: 2022-10-11 Impact factor: 2.809