Baoxiang Zhang1, Tingting Li1, Yuanyuan Tang2, Mao Lin3, Caixia Tu4, Yong Lang5, Diancai Zhang1, Dianqin Feng1. 1. Department of Dermatology, Yidu Central Hospital, Weifang Medical University, Qingzhou, China. 2. Department of Division of Rheumatology, Yidu Central Hospital, Weifang Medical University, Qingzhou, China. 3. Department of Dermatology, Chongqing Chinese Medicine Hospital, Chongqing, China. 4. Department of Dermatology, The 2nd Affiliated Hospital of Dalian Medical University, Dalian, China. 5. Department of Dermatology, Gaomi People's Hospital, Gaomi, China.
Abstract
BACKGROUND: To explore the associations between this treatment and CD4+, CD8+ T-cells, and regulatory T cells (Treg). METHODS: We collected the skin biopsies from 295 patients with nonsegmental vitiligo treated with or without 308-nm excimer laser. We revealed that patients at stable stage showed a better response to 308-nm excimer laser than those at active stage. RESULTS: In comparison with untreated patients, CD4+ and CD8+ T cells were both reduced while Foxp3+ Treg cells, TGF-β, and IL-10 were elevated in the lesional sites of patients who showed effective reaction to the treatment. In the treated patients at active stage, the infiltration of CD4+ and CD8+ T cells was significantly declined but Foxp3+ Treg cells, TGF-β, and IL-10 were increased compared to those in untreated patients at active stage. These changes of cell infiltration were more obvious in the treated patients at stable stage, explaining why there were more patients at stable stage response better than patients at active stage. CONCLUSIONS: 308-nm excimer laser is effective to reduce the infiltration of CD4+ and CD8+ T cells but promote the infiltration Treg cells and secretion of TGF-β and IL-10 in the lesion skin of vitiligo patients, especially at stable stage.
BACKGROUND: To explore the associations between this treatment and CD4+, CD8+ T-cells, and regulatory T cells (Treg). METHODS: We collected the skin biopsies from 295 patients with nonsegmental vitiligo treated with or without 308-nm excimer laser. We revealed that patients at stable stage showed a better response to 308-nm excimer laser than those at active stage. RESULTS: In comparison with untreated patients, CD4+ and CD8+ T cells were both reduced while Foxp3+ Treg cells, TGF-β, and IL-10 were elevated in the lesional sites of patients who showed effective reaction to the treatment. In the treated patients at active stage, the infiltration of CD4+ and CD8+ T cells was significantly declined but Foxp3+ Treg cells, TGF-β, and IL-10 were increased compared to those in untreated patients at active stage. These changes of cell infiltration were more obvious in the treated patients at stable stage, explaining why there were more patients at stable stage response better than patients at active stage. CONCLUSIONS: 308-nm excimer laser is effective to reduce the infiltration of CD4+ and CD8+ T cells but promote the infiltration Treg cells and secretion of TGF-β and IL-10 in the lesion skin of vitiligo patients, especially at stable stage.