| Literature DB >> 31670034 |
Nicole Silbernagel1, Arlene Körner1, Jakob Balitzki1, Mona Jaggy2, Sarah Bertels2, Benjamin Richter3, Marc Hippler3, Andrea Hellwig4, Markus Hecker5, Martin Bastmeyer6, Nina D Ullrich7.
Abstract
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) represent the best cell source for cardiac regenerative purposes but retain an immature phenotype after differentiation with significant limitations compared to adult cardiomyocytes. Apart from an incomplete cardiomyocyte-specific structure and microarchitecture, cells show at the level of Ca2+ signaling only slow Ca2+ release and reuptake properties. Here, we investigated the effect of restructuring single iPSC-CMs in specially designed 3D-micro-scaffolds on cell morphology and Ca2+ handling. Using direct laser writing, rectangular-shaped scaffolds were produced and single iPSC-CMs were seeded into these forms. Structural analyses revealed strong sarcolemmal remodeling processes and myofilament reorientation in 3D-shaped cells leading to enhanced clustered expression of L-type Ca2+ channels and ryanodine receptors and consequently, to faster Ca2+ transient kinetics. Spontaneous beating activity was enhanced and Ca2+ handling was more robust compared to non-patterned cells. Overall, our data demonstrate for the first time significant improvement of Ca2+ signaling properties in reshaped iPSC-CMs indicative of functional maturation by structural remodeling.Entities:
Keywords: 3D-micro-scaffolds; Ca(2+) signaling; Direct laser writing; Maturation; Structure-function; iPSC cardiomyocytes
Mesh：
Year: 2019 PMID： 31670034 DOI： 10.1016/j.biomaterials.2019.119551
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479