Karthika Muthuramalingam1, Vineet Singh2, Changmin Choi1, Seung In Choi3, Young Mee Kim1, Tatsuya Unno4,5, Moonjae Cho6,7,8. 1. Department of Biochemistry, School of Medicine, Jeju National University, Jeju, 63243, Republic of Korea. 2. Faculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju, 63243, Republic of Korea. 3. Department of Pharmaceutical Research Institute, Quegen Biotech Co. Ltd, Seoul, 429931, Republic of Korea. 4. Faculty of Biotechnology, School of Life Sciences, SARI, Jeju National University, Jeju, 63243, Republic of Korea. tatsu@jejunu.ac.kr. 5. Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, 63243, Republic of Korea. tatsu@jejunu.ac.kr. 6. Department of Biochemistry, School of Medicine, Jeju National University, Jeju, 63243, Republic of Korea. moonjcho@jejunu.ac.kr. 7. Institute of Medical Science, Jeju National University, Jeju, 63241, Republic of Korea. moonjcho@jejunu.ac.kr. 8. Interdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju, 63241, Republic of Korea. moonjcho@jejunu.ac.kr.
Abstract
PURPOSE: Western diet, rich in carbohydrates and fat, is said to be a major factor underlying metabolic syndrome. Interventions with prebiotics, the key modulators of the gut microbiota, have paramount impact on host-associated metabolic disorders. Herein, we investigated the effect of fungus-derived (1,3)/(1,6)-β-glucan, a highly soluble dietary fiber, on high-fat diet (HFD)-induced metabolic distress. METHODS: Male C57BL/6 J mice were fed with different diet groups (n = 11): control diet, HFD, 3 g/kg or 5 g/kg of β-glucan-incorporated HFD. At the end of experimental study period (12th week), body weight, feces weight and fecal moisture content were observed. Further, colonic motility was measured using activated charcoal meal study. Proteins extracted from liver and intestine tissues were subjected to western blot technique. Paraffin-embedded intestinal tissues were sectioned for histochemical [Periodic acid-Schiff (PAS) and Alcian blue (AB) staining] analysis. Fecal microbiota analysis was performed using MOTHUR bioinformatic software. RESULTS: β-glucan consumption exhibited anti-obesity property in mice groups fed with HFD. In addition, β-glucan ameliorated HFD-induced hepatic stress, colonic motility and intestinal atrophy (reduction in colon length, goblet cells, and mucosal layer thickness). Further, β-glucan incorporation shifted bacterial community by increasing butyrate-producing bacteria such as Anaerostipes, Coprobacillus, and Roseburia and decreasing reportedly obesity-associated bacteria such as Parabacteroides and Lactococcus. CONCLUSION: Altogether, the outcomes of this present pre-clinical animal study show β-glucan to be a promising therapeutic candidate in the treatment of HFD-induced metabolic distress. Further comprehensive research has to be conducted to brace its clinical relevance, reproducibility and efficacy for aiding human health.
PURPOSE: Western diet, rich in carbohydrates and fat, is said to be a major factor underlying metabolic syndrome. Interventions with prebiotics, the key modulators of the gut microbiota, have paramount impact on host-associated metabolic disorders. Herein, we investigated the effect of fungus-derived (1,3)/(1,6)-β-glucan, a highly soluble dietary fiber, on high-fat diet (HFD)-induced metabolic distress. METHODS: Male C57BL/6 J mice were fed with different diet groups (n = 11): control diet, HFD, 3 g/kg or 5 g/kg of β-glucan-incorporated HFD. At the end of experimental study period (12th week), body weight, feces weight and fecal moisture content were observed. Further, colonic motility was measured using activated charcoal meal study. Proteins extracted from liver and intestine tissues were subjected to western blot technique. Paraffin-embedded intestinal tissues were sectioned for histochemical [Periodic acid-Schiff (PAS) and Alcian blue (AB) staining] analysis. Fecal microbiota analysis was performed using MOTHUR bioinformatic software. RESULTS: β-glucan consumption exhibited anti-obesity property in mice groups fed with HFD. In addition, β-glucan ameliorated HFD-induced hepatic stress, colonic motility and intestinal atrophy (reduction in colon length, goblet cells, and mucosal layer thickness). Further, β-glucan incorporation shifted bacterial community by increasing butyrate-producing bacteria such as Anaerostipes, Coprobacillus, and Roseburia and decreasing reportedly obesity-associated bacteria such as Parabacteroides and Lactococcus. CONCLUSION: Altogether, the outcomes of this present pre-clinical animal study show β-glucan to be a promising therapeutic candidate in the treatment of HFD-induced metabolic distress. Further comprehensive research has to be conducted to brace its clinical relevance, reproducibility and efficacy for aiding human health.
Entities:
Keywords:
Gut microbiota; High-fat diet; Metabolic syndrome; Obesity; Prebiotics; β-glucan
Authors: T Z DeSantis; P Hugenholtz; N Larsen; M Rojas; E L Brodie; K Keller; T Huber; D Dalevi; P Hu; G L Andersen Journal: Appl Environ Microbiol Date: 2006-07 Impact factor: 4.792
Authors: Patrick D Schloss; Sarah L Westcott; Thomas Ryabin; Justine R Hall; Martin Hartmann; Emily B Hollister; Ryan A Lesniewski; Brian B Oakley; Donovan H Parks; Courtney J Robinson; Jason W Sahl; Blaz Stres; Gerhard G Thallinger; David J Van Horn; Carolyn F Weber Journal: Appl Environ Microbiol Date: 2009-10-02 Impact factor: 4.792
Authors: Andrew J Holmes; Yi Vee Chew; Feyza Colakoglu; John B Cliff; Eline Klaassens; Mark N Read; Samantha M Solon-Biet; Aisling C McMahon; Victoria C Cogger; Kari Ruohonen; David Raubenheimer; David G Le Couteur; Stephen J Simpson Journal: Cell Metab Date: 2016-11-23 Impact factor: 27.287
Authors: Maria C Della Vedova; Marcos D Muñoz; Lucas D Santillan; Maria G Plateo-Pignatari; Maria J Germanó; Martín E Rinaldi Tosi; Silvina Garcia; Nidia N Gomez; Miguel W Fornes; Sandra E Gomez Mejiba; Dario C Ramirez Journal: Nutr Metab Insights Date: 2016-12-05