Eslam Essam Mohammed1, Sema Yilmaz2, Oya Alagoz Akcin3, Barbaros Nalbantoglu1, Cem Ficicioglu3, Fikrettin Sahin4, Esra Aydemir Coban5. 1. Department of Biochemistry, Yildiz Technical University, Istanbul, Turkey. 2. Division of Pediatric Hematology/Oncology, Health Science University Kartal Lutfi Kirdar Education and Research Hospital, Istanbul, Turkey. 3. Department of Gynecology and Obstetrics, Yeditepe University Faculty of Medicine, Istanbul, Turkey. 4. Department of Bioengineering and Genetics, Yeditepe University Faculty of Medicine, Istanbul, Turkey. 5. Department of Bioengineering and Genetics, Yeditepe University Faculty of Medicine, Istanbul, Turkey esra.aydemir@yeditepe.edu.tr.
Abstract
BACKGROUND/AIM: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells. MATERIALS AND METHODS: CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days. RESULTS: Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle. CONCLUSION: CCs conditioned medium induced apoptosis of pancreatic cancer cells. Copyright
BACKGROUND/AIM: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells. MATERIALS AND METHODS: CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3pancreatic cancer cells were treated with CC conditioned media for three days. RESULTS: Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle. CONCLUSION: CCs conditioned medium induced apoptosis of pancreatic cancer cells. Copyright
Authors: Ana Gvozdenovic; Matthias J E Arlt; Carmen Campanile; Patrick Brennecke; Knut Husmann; Yufei Li; Walter Born; Roman Muff; Bruno Fuchs Journal: J Bone Miner Res Date: 2013-04 Impact factor: 6.741