Literature DB >> 3166132

Expression of tandem gene fusions in transgenic tobacco plants.

C Dean1, M Favreau, S Tamaki, D Bond-Nutter, P Dunsmuir, J Bedbrook.   

Abstract

We have studied the expression of four sets of tandem gene fusions in transgenic tobacco plants. This was to determine if the problem of between-transformant variability in expression of introduced genes could be overcome by using a linked reference gene as a co-ordinately expressed control. Tandem gene fusions containing identical 5' flanking regions (SSU301-ocs with either SSU301-cat or SSU301-SSU911) were not co-ordinately expressed in the transgenic tobacco plants whereas the tandem gene fusions containing similar but not identical 5' flanking regions (SSU301-ocs with SSU911-cat or SSU911-SSU301) were co-ordinately expressed. The lack of co-ordinate expression of some of the tandem gene fusions appears to be partially explained by absence of the corresponding genomic DNA segments in the transgenic plants.

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Year:  1988        PMID: 3166132      PMCID: PMC338429          DOI: 10.1093/nar/16.15.7601

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  15 in total

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2.  Organ-specific and light-induced expression of plant genes.

Authors:  R Fluhr; C Kuhlemeier; F Nagy; N H Chua
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3.  Confirmation of the relative expression levels of the Petunia (Mitchell) rbcS genes.

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4.  Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.

Authors:  D P Taylor; S N Cohen
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

5.  Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9.

Authors:  N K Alton; D Vapnek
Journal:  Nature       Date:  1979 Dec 20-27       Impact factor: 49.962

6.  Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells.

Authors:  G An
Journal:  Plant Physiol       Date:  1986-05       Impact factor: 8.340

7.  Selection-expression plasmid vectors for use in genetic transformation of higher plants.

Authors:  J Velten; J Schell
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

8.  Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene.

Authors:  H De Greve; P Dhaese; J Seurinck; M Lemmers; M Van Montagu; J Schell
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9.  Sequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.

Authors:  C Kuhlemeier; R Fluhr; P J Green; N H Chua
Journal:  Genes Dev       Date:  1987-05       Impact factor: 11.361

10.  Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family.

Authors:  C Dean; P Elzen; S Tamaki; P Dunsmuir; J Bedbrook
Journal:  EMBO J       Date:  1985-12-01       Impact factor: 11.598

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  11 in total

1.  Quantitation of chloramphenicol acetyl transferase in transgenic tobacco plants by ELISA and correlation with gene copy number.

Authors:  E H Gendloff; B Bowen; W G Buchholz
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2.  Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters.

Authors:  C Peach; J Velten
Journal:  Plant Mol Biol       Date:  1991-07       Impact factor: 4.076

3.  Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter.

Authors:  M Sanger; S Daubert; R M Goodman
Journal:  Plant Mol Biol       Date:  1990-03       Impact factor: 4.076

4.  Sulfonamide resistance gene for plant transformation.

Authors:  F Guerineau; L Brooks; J Meadows; A Lucy; C Robinson; P Mullineaux
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

5.  High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco.

Authors:  G C Allen; G Hall; S Michalowski; W Newman; S Spiker; A K Weissinger; W F Thompson
Journal:  Plant Cell       Date:  1996-05       Impact factor: 11.277

6.  Sequences 5' to translation start regulate expression of petunia rbcS genes.

Authors:  C Dean; M Favreau; J Bedbrook; P Dunsmuir
Journal:  Plant Cell       Date:  1989-02       Impact factor: 11.277

7.  Sequences downstream of translation start regulate quantitative expression of two petunia rbcS genes.

Authors:  C Dean; M Favreau; D Bond-Nutter; J Bedbrook; P Dunsmuir
Journal:  Plant Cell       Date:  1989-02       Impact factor: 11.277

8.  Coordinate expression of antibody subunit genes yields high levels of functional antibodies in roots of transgenic tobacco.

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Journal:  Plant Mol Biol       Date:  1994-12       Impact factor: 4.076

9.  Hairy roots - a short cut to transgenic root nodules.

Authors:  J Hansen; J E Jørgensen; J Stougaard; K A Marcker
Journal:  Plant Cell Rep       Date:  1989-05       Impact factor: 4.570

10.  Expression of the isopentenyl transferase gene is regulated by auxin in transgenic tobacco tissues.

Authors:  X D Zhang; D S Letham; R Zhang; T J Higgins
Journal:  Transgenic Res       Date:  1996-01       Impact factor: 2.788

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