| Literature DB >> 31660951 |
Xin Xia1,2, Xixi Li1,2, Fanying Li1,2, Xujia Wu1,2, Maolei Zhang1,2, Huangkai Zhou1,2, Nunu Huang1,2, Xuesong Yang1,2, Feizhe Xiao3, Dawei Liu4, Lixuan Yang1,2, Nu Zhang5,6.
Abstract
In the published article [1], an error was noticed in Fig. 6B. The western blot results were reversed between the overexpression group and the knockdown group of circ-AKT3. The corrected and updated Fig. 6 is provided below. This error does not affect the findings or conclusions of the article.Entities:
Year: 2019 PMID: 31660951 PMCID: PMC6819491 DOI: 10.1186/s12943-019-1083-2
Source DB: PubMed Journal: Mol Cancer ISSN: 1476-4598 Impact factor: 27.401
Fig. 6AKT3-174aa competitively interacts with p-PDK1 form ATK2/3. a PDK1 kinase activity was determined in U251 and U373 cells with circ-AKT3 overexpression and their control cells at the indicated time point. Error bars represent three independent experiments, **, p < 0.01. b Upper, AKT kinase activity was determined in U251 and U373 cells with circ-AKT3 overexpression and their control cells (48 h); or in SW1783 and Hs683 cells with circ-AKT3 knocking down and their control cells (48 h). Error bars represent three independent experiments, *, p < 0.05, **, p < 0.01. Lower, p-SGK level was determined in indicated cells. c U251 cells were transfected with increasingly dose of circ-AKT3. IP was performed by using AKT2, AKT3 and p-PDK1 antibodies and followed by immunoblot using indicated antibodies. d SW1783 cells were transfected with increasingly dose of circ-AKT3 shRNA. IP was performed by using AKT2, AKT3 and p-PDK1 antibodies and followed by immunoblot using indicated antibodies