Literature DB >> 3165980

A microtiter cell-culture assay for the determination of anti-human immunodeficiency virus neutralizing antibody activity.

G A Robertson1, B M Kostek, W A Schleif, J A Lewis, E A Emini.   

Abstract

A microtiter cell-culture assay is described for measuring neutralizing antibody activity directed against the human immunodeficiency virus (HIV). The assay relies upon inhibition of HIV-mediated cell killing of infected MT-4 lymphoid cells. The assay exhibits comparable sensitivity to two other methods used for such measurements, is relatively rapid, may be adapted for screening large numbers of samples and involves minimal handling of infectious virus.

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Year:  1988        PMID： 3165980     DOI： 10.1016/0166-0934(88)90123-1

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  11 in total

1.  Examination of sera from human immunodeficiency virus type 1 (HIV-1)-infected individuals for antibodies reactive with peptides corresponding to the principal neutralizing determinant of HIV-1 gp120 and for in vitro neutralizing activity.

Authors:  R Q Warren; S A Anderson; W M Nkya; J F Shao; C W Hendrix; G P Melcher; R R Redfield; R C Kennedy
Journal:  J Virol       Date:  1992-09       Impact factor: 5.103

2.  Characterization and large production of human monoclonal antibodies against the HIV-1 envelope.

Authors:  V Boyer; H Broly; S Souche; P Madaule; J Rossier; D Zagury; C Desgranges
Journal:  Clin Exp Immunol       Date:  1991-03       Impact factor: 4.330

3.  WST-1 assay--a simple colorimetric method for virus titration.

Authors:  E Nissen; G Pauli; D Vollenbroich
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-01       Impact factor: 2.416

4.  Application of a rapid microplaque assay for determination of human immunodeficiency virus neutralizing antibody titers.

Authors:  C V Hanson; L Crawford-Miksza; H W Sheppard
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

5.  Agreement study between two laboratories of immunofluorescence as a confirmatory test for human immunodeficiency virus type 1 antibody screening.

Authors:  J Mahony; K Rosenthal; M Chernesky; S Castriciano; E Scheid; M Blajchman; D Harnish
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

6.  Comparison of antibody reactivity to human immunodeficiency virus type 1 (HIV-1) gp160 epitopes in sera from HIV-1-infected individuals from Tanzania and from the United States.

Authors:  R Q Warren; W M Nkya; J F Shao; S A Anderson; H Wolf; C W Hendrix; P Kanda; M Wabuke; R N Boswell; R R Redfield
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

7.  Neutralization of diverse human immunodeficiency virus type 1 variants by an anti-V3 human monoclonal antibody.

Authors:  M K Gorny; A J Conley; S Karwowska; A Buchbinder; J Y Xu; E A Emini; S Koenig; S Zolla-Pazner
Journal:  J Virol       Date:  1992-12       Impact factor: 5.103

8.  Adaptation to persistent growth in the H9 cell line renders a primary isolate of human immunodeficiency virus type 1 sensitive to neutralization by vaccine sera.

Authors:  T Wrin; T P Loh; J C Vennari; H Schuitemaker; J H Nunberg
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

9.  Neutralization of divergent human immunodeficiency virus type 1 variants and primary isolates by IAM-41-2F5, an anti-gp41 human monoclonal antibody.

Authors:  A J Conley; J A Kessler; L J Boots; J S Tung; B A Arnold; P M Keller; A R Shaw; E A Emini
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

10.  Antibody-mediated in vitro neutralization of human immunodeficiency virus type 1 abolishes infectivity for chimpanzees.

Authors:  E A Emini; P L Nara; W A Schleif; J A Lewis; J P Davide; D R Lee; J Kessler; S Conley; S Matsushita; S D Putney
Journal:  J Virol       Date:  1990-08       Impact factor: 5.103
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