Determinants of adverse neonatal outcomes among postnatal women in Dar es Salaam -Tanzania.

BACKGROUND: Neanatal comploications are the commonest problems facing babies in Tanzania. An attempt has been made to investigate determinants of neonatal outcomes among postnatal women.
METHODS: Using a case-contrrol study design, 165 women were selected from 3 health facilities, where they had had antenatal care (ANC), labour, delivery and post natal care.
RESULTS: Chi- square test revealed that gestational age (p-value, 0.01), HIV status (p-value, 0.000) and malaria (p-value, 0.001<0.05) were significantly associated with adverse neonatal outcomes.
CONCLUSION: The study concluded that implementation of community-based intervention is needed to ensure survival of newborns.

Background

Globally, it is recognized that approximately thirty millions new borns worldwide are characterized with low birth weight and hence becoming one of the leading determinant of mortality and disability1. This implies that weight at birth is an important indicator of mortality both at individual and community level. Adverse neonatal outcomes such as death, low birth weight (LBW) and preterm birth are common in developing countries including Tanzania2. In most sub-Saharan countries including Tanzania, HIV infection has become a leading medical complication of pregnancy and cause of maternal and neonatal morbidity and mortality3. Fortunately, anti-retroviral medication and good obstetric practice have greatly reduced both maternal death and transmission of HIV infection to the infant4. It has been reported that low birth weight, prematurity, and infection including HIV, are the main factors that contribute to neonatal mortality5,6,7. Low birth weight remains a public health problem in many parts of the world and is associated with a range of health problems, lasting disabilities and even death8.

Tanzania is making efforts in reducing child mortality, but has demonstrated slower progress due to high levels of neonatal outcomes9. About 51,000 newborns die each year, which places it among the top five countries with the most newborn deaths in sub-Saharan Africa10,11.

According to a study by Xiao et al12 in China, it was concluded that no significant relationship exists between maternal HIV infection and adverse pregnancy outcomes. In Canada, Macdonald et al13 concluded that women living with HIV were more likely to undergo caesarean section and deliver preterm. In United State of America, HIV infected women with concomitant STI's were reported to have a higher risk of spontaneous preterm birth.14 Mitgitti et al15 in Thailand reported that low birth weight was associated with positive maternal HIV status.

The studies conducted in Nigeria16 and South Africa17 reported that adverse event of low birth weight, preterm delivery retained independent association with HIV infection. 18 In Tanzania, it is documented that HIV infected women are at a higher risk of adverse pregnancy outcomes19,20,21

The existing studies in Tanzania were designed on site specific, targeting specific groups and none has utilized routine Health Management Information System (HMIS) data. Thus this study focused on investigating determinants of neonatal outcomes among postnatal women. The key strength of this paper is on examining the general neonatal outcomes including low birth weight (LBW), infant mortality and preterm, unlike the existing papers that focused on a either LBW alone, infant mortality or preterm.

Materials and methods

Research design

This was an observational study that employed a case control design. Cases were all the adverse neonatal outcomes, while the control group were women without neonatal outcomes. The cases were recruited from health centers; control group was similarly selected from a population of health centers.

Area of study

The study was conducted at Ilala which is one of the five municipals found in Dar es Salaam region of Tanzania. The study involved three public health facilities namely Hospital, Health Centre and Dispensary.

Target population and Outcome of interest

Target population was the cohort of all women who received; ANC, labor and delivery and postnatal care in the health facilities between January 2014 to November 2016. The outcome variable was the adverse neonatal outcome defined as a newborn from a selected case or control with any of the following outcome, low birth weight, born prematurity, low Apgar score or death and still birth (fresh and macerated).

Sample and sampling procedure

Sample of the study

A sample is the sub-group of the population which should be representative in the sense that each sampled unit represents the characteristics of the known number of a unit in the population21. Sample size was obtained by u sing the formula developed by Kelsey22 given by;

Where

n2 = rn1

n1 = Number of cases

n2 = Number of controls

Standard normal deviate for two tailed test based on alpha level (related to confidence interval level).

Standard normal deviate for one tailed test based on beta level (related to the power level)

Ratio of controls to cases

The sample size calculation considered a two-sided confidence level at 95% (α=5 %), Zα/2 = Z0.25 = 1.96) and a power (% chance of detecting) of 80% (Z1-β = z0.2 = 0.845). The ratio of controls to cases was taken to be 4.Thus the actual sample size was obtained as follows;

n2= rn1 = 4(33) =132

The minimal sample size needed for the study was estimated to be 33+132=165

Sampling procedure

This study used probability sampling procedure particularly simple random sampling techniques to select cases and controls. Using the registers for ANC, delivery and Postnatal, two separate lists of cases and controls was made (for the defined period) to develop sampling frame for each. The list of patients with their profiles was assigned random number and finally the targeted sample size was easy selected using SPSS. Since the study employed the secondary data that had already been recorded, the information of the individuals selected were taken for this analysis. Selection started by cases to obtain the required sample, then controls were selected afterwards.

Data collection method

This study employed secondary data available at health facilities which were primarily collected using a questionnaire that contained variables from three registers (i.e. ANC, labor and delivery and postnatal). The information retrieved from the recorded data includes HIV and malaria status, age of mother, gestational age, parity factors, number of pregnancies, history of stillbirth, parity level, anemia and blood pressure status.

Statistical model

Chi-squared test was used to determine the association between variables, and binary logistic regression model to check the influence of variables to the occurrence of adverse neonatal outcomes. In logistic regression, the probability of Y occurring given known values of Xi is predicted as;

Where i=1, 2, 3………..165 and j=1, 2…5

Where πi is the probability of respondent i, Xij are different variables associating with adverse neonatal outcomes namely HIV status, clinical factors, parity factors, sociodemographic factors and gestation age, βj stands for the parameters and β0 is a constant term.

Results

Association between neonatal outcomes and several factors Chi- squared test was used to test association between each factor and combined indicators of adverse neonatal outcomes. In checking whether demographic factors particularly age of mother are related to the neonatal outcome, the results revealed insignificant relationship (p-value of 0.204 > 0.05). Contrary to the findings on mother's age, gestational age was significant linked to adverse of neonatal outcomes as evidenced with its corresponding p-value of 0.01<0.05.Simialry, the HIV status was highly significant associated with the occurrence of neonatal outcomes (p-value, 0.000<0.05).

Three parity factors namely parity levels, previous number of pregnancy and history of still birth were tested for significance association. Results indicate that parity level (p-value, 0.941>0.05), previous number of pregnancy (p-value 0.378>0.05) and history of still birth (p-value, 0.13>0.05) are not associated with adverse neonatal outcomes. In the same way, three clinical factors particularly anemia status, blood pressure and malaria infection of a mother were also tested. Findings show that anemia (p-value, 0.535>0.05) and blood pressure (p-value, 0.316>0.05) had no relationship with adverse neonatal outcomes. However, there was a significant association between malaria infection and neonatal outcomes (p-value, 0.001<0.05).

Discussion

This study showed that malaria during pregnancy is significantly associated with neonatal outcome (p -value 0.001), and this implies that infected women with malaria during pregnancy had a high possibility of delivering newborn with neonatal outcome. This result was similar to those of previous studies in which malaria in pregnancy was highly related with preventable low birth weight of newborn in women of all pregnancy orders in sub-Saharan Africa22. This result implies that despite several efforts made to minimize the burden of malaria in pregnancy, relationship between malaria and neonatal outcome particularly infant mortality is stable. This study revealed association of adverse neonatal outcomes and maternal HIV infection. This is consistent with study in South Africa which found that HIV infected women and adverse outcome were significant23. A study conducted in Nigeria24 concluded that infected women are prevalent in Nigeria and it is highly associated with infant low birth weight. It is also similar with the past studies conducted in other parts of African continent25,26, including studies conducted in Tanzania27. While the results indicate a non statistical significance association between neonatal outcomes and parity factors, the situation was quite different to the case of gestational age where by its association with neonatal outcomes was significant. This finding concurs with the past study conducted in Tanzania28. Multiple pregnancies were not significantly associated with neonatal outcome unlike the past studies29,30 which reported significant relationship.

Despite the fact that anemia during pregnancy is well recognized as among one of the public problem that significantly contributes to the neonatal outcomes, the finding shows no significant association with neonatal outcomes. This finding contrasted with those of other studies31. Similarly the past studies revealed a significant association between age of the mother and neonatal outcome32, the current study did not show significant association. The main reason of this contract is due to inadequate sample size which lowers the power of the statistical test. With large sample and even population based studies, the results might be quite different. This limitation of the study that should be considered when we need to generalize the results

Conclusion

With good antenatal care and multidisciplinary approach, HIV-infected women can ensure quality care of mothers and newborns. In order to increase infant survival, implementation of community-based intervention is also needed and should address basic education, prevention of malaria in pregnancy and poverty eradication. Similarly to optimize the survival of children, the key objective of perinatal research should focus on minimizing the incidence of neonatal outcomes.

Table 1

Association between neonatal outcomes and various factors

Factors	Subcategory	Neonatal outcomes			p-value
		No	Yes	Total
Mothers age	18–25years	27	29	56	0.204
	26–34 years	28	54	82
	35+ years	9	18	27
Gestation age	1st-trimester	6	13	19	0.01
	2nd-trimester	51	59	110
	3rd-trimester	7	29	36
Parity level	0_Parity	15	26	41	0.941
	1_Parity	22	33	55
	>1_Parity	27	42	69
Pregnancy	1st_Pregnancy	13	23	36	0.378
	2–3_Pregnancies	39	51	90
	>3_Pregnancies	12	27	39
History of Stillbirth	Never	56	79	135	0.13
	At least 1	8	22	30
HIV status	HIV negative	55	77	132	0.000
	HIV positive	1	32	33
Anemia	Normal	38	55	93	0.535
	Anemic	26	46	72
Malaria	Negative	61	58	119	0.001
	Positive	3	43	46
Blood pressure	Abnormal	46	65	111	0.316
	Normal	18	36	54