Rehan Abdur Rab1, Sultan Zahiruddin2, Mohammad Ibrahim2, Faisal Husain3, Rabea Parveen4, Washim Khan2, Farhan J Ahmad1, Asim A Khan5, Sayeed Ahmad2. 1. Jamia Hamdard, School of Pharmaceutical Education and Research, Department of Pharmaceutics, Nano Medicine Laboratory, New Delhi 110062, India. 2. Jamia Hamdard, School of Pharmaceutical Education and Research, Department of Pharmacognosy and Photochemistry, Bioactive Natural Product Laboratory, New Delhi 110062, India. 3. Rehbar Ayurvedic and Unani Medical College, Bhawanigarh, Punjab 148026, India. 4. Jamia Millia Islamia, Department of Biosciences, New Delhi 110025, India. 5. Ministry of AYUSH, Central Council for Research in Unani Medicine, New Delhi 110058, India.
Abstract
BACKGROUND: Ultra-performance LC (UPLC)-tandem MS (MS/MS) and high-performance TLC (HPTLC) assay methods were developed for chemical fingerprinting and quantitative analysis of bioactive constituents of a certain "Itrifal formulation," a traditional kind of Unani medicine. OBJECTIVE: In the present investigation, HPTLC and UPLC-MS/MS methods were developed and validated for the detection and quantification of major metabolites present in itrifal formulation. METHODS: The metabolites present in the formulation were separated using modern chromatographic techniques, and a quantitative analysis was performed. Analytical performance of the proposed HPTLC and UPLC-MS/MS methods was validated as per the defined guidelines with respect to linearity, accuracy, precision, robustness, and specificity. RESULTS: The developed UPLC-MS/MS and HPTLC methods were used for quantification of gallic acid, tannic acid, catechin, and quercetin. All four constituents were quantified by UPLC-MS/MS, while two constituents were quantified by HPTLC in the commercial itrifal formulation. The calibration plot was found to be linear, accurate, precise, robust, and specific for both HPTLC and UPLC-MS/MS. CONCLUSIONS: The present methods were successfully applied for analysis of the given markers in itrifal formulations. The same can be used for QC and stability testing of itrifal formulations.
BACKGROUND: Ultra-performance LC (UPLC)-tandem MS (MS/MS) and high-performance TLC (HPTLC) assay methods were developed for chemical fingerprinting and quantitative analysis of bioactive constituents of a certain "Itrifal formulation," a traditional kind of Unani medicine. OBJECTIVE: In the present investigation, HPTLC and UPLC-MS/MS methods were developed and validated for the detection and quantification of major metabolites present in itrifal formulation. METHODS: The metabolites present in the formulation were separated using modern chromatographic techniques, and a quantitative analysis was performed. Analytical performance of the proposed HPTLC and UPLC-MS/MS methods was validated as per the defined guidelines with respect to linearity, accuracy, precision, robustness, and specificity. RESULTS: The developed UPLC-MS/MS and HPTLC methods were used for quantification of gallic acid, tannic acid, catechin, and quercetin. All four constituents were quantified by UPLC-MS/MS, while two constituents were quantified by HPTLC in the commercial itrifal formulation. The calibration plot was found to be linear, accurate, precise, robust, and specific for both HPTLC and UPLC-MS/MS. CONCLUSIONS: The present methods were successfully applied for analysis of the given markers in itrifal formulations. The same can be used for QC and stability testing of itrifal formulations.