Literature DB >> 3165082

Strong transcriptional promoter in the 5' upstream region of the human beta-actin gene.

H Sugiyama1, H Niwa, K Makino, T Kakunaga.   

Abstract

We isolated the 4.3-kb nucleotide sequence 5' upstream from the coding region of the human beta-actin gene and tested its promoter function by using the chloramphenicol acetyl transferase assay. We found that the sequence required for efficient transcription was contained within the 1.2-kb DNA fragment of the actin 5'-noncoding region. The 1.2-kb fragment contained a TATA box, a CCAAT box and two highly conserved elements. The transcriptional start point was located 24 bp downstream from the TATA box. The 1.2-kb fragment showed strong promoter activity when it was transfected into human and rodent non-muscle cells. In human FL cells and in mouse NIH3T3 and L cells, the promoter activity of the 1.2-kb DNA fragment was greater than that of the SV40 early region.

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Year:  1988        PMID： 3165082     DOI： 10.1016/0378-1119(88)90426-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  9 in total

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