Literature DB >> 31649967

Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal mouse Macrophages by Flow Cytometry.

Katharina Richard1, Stefanie N Vogel1, Darren J Perkins1.   

Abstract

Internalization of cell surface Toll Like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.

Entities:  

Keywords:  CD14; Endocytosis; LPS; TLR4; TRIF; peritoneal macrophages

Year:  2019        PMID： 31649967      PMCID： PMC6812529          DOI： 10.21769/BioProtoc.3369

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  CD14 controls the LPS-induced endocytosis of Toll-like receptor 4.

Authors:  Ivan Zanoni; Renato Ostuni; Lorri R Marek; Simona Barresi; Roman Barbalat; Gregory M Barton; Francesca Granucci; Jonathan C Kagan
Journal:  Cell       Date:  2011-11-11       Impact factor: 41.582

Review 2.  TLR signaling pathways.

Authors:  Kiyoshi Takeda; Shizuo Akira
Journal:  Semin Immunol       Date:  2004-02       Impact factor: 11.130

3.  Chemical, physical, biological properties of a lipopolysaccharide from Escherichia coli K-235.

Authors:  F C McIntire; H W Sievert; G H Barlow; R A Finley; A Y Lee
Journal:  Biochemistry       Date:  1967-08       Impact factor: 3.162

4.  CD14 dependence of TLR4 endocytosis and TRIF signaling displays ligand specificity and is dissociable in endotoxin tolerance.

Authors:  Rajesh Rajaiah; Darren J Perkins; Derek D C Ireland; Stefanie N Vogel
Journal:  Proc Natl Acad Sci U S A       Date:  2015-06-23       Impact factor: 11.205

5.  Thermodynamics of transfer of cholesterol from gel to fluid phases of phospholipid bilayers.

Authors:  C H Spink; S Manley; M Breed
Journal:  Biochim Biophys Acta       Date:  1996-03-13

6.  TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta.

Authors:  Jonathan C Kagan; Tian Su; Tiffany Horng; Amy Chow; Shizuo Akira; Ruslan Medzhitov
Journal:  Nat Immunol       Date:  2008-02-24       Impact factor: 25.606

7.  Phosphatidylcholine: cholesterol phase diagrams.

Authors:  J L Thewalt; M Bloom
Journal:  Biophys J       Date:  1992-10       Impact factor: 4.033

8.  Autocrine-paracrine prostaglandin E2 signaling restricts TLR4 internalization and TRIF signaling.

Authors:  Darren J Perkins; Katharina Richard; Anne-Marie Hansen; Wendy Lai; Shreeram Nallar; Beverly Koller; Stefanie N Vogel
Journal:  Nat Immunol       Date:  2018-11-05       Impact factor: 25.606
  8 in total
  2 in total

1.  Dissociation of TRIF bias and adjuvanticity.

Authors:  Katharina Richard; Darren J Perkins; Erin M Harberts; Yang Song; Archana Gopalakrishnan; Kari Ann Shirey; Wendy Lai; Alexandra Vlk; Anup Mahurkar; Shreeram Nallar; Lynn D Hawkins; Robert K Ernst; Stefanie N Vogel
Journal:  Vaccine       Date:  2020-05-07       Impact factor: 3.641

2.  A mouse model of human TLR4 D299G/T399I SNPs reveals mechanisms of altered LPS and pathogen responses.

Authors:  Katharina Richard; Kurt H Piepenbrink; Kari Ann Shirey; Archana Gopalakrishnan; Shreeram Nallar; Daniel J Prantner; Darren J Perkins; Wendy Lai; Alexandra Vlk; Vladimir Y Toshchakov; Chiguang Feng; Rachel Fanaroff; Andrei E Medvedev; Jorge C G Blanco; Stefanie N Vogel
Journal:  J Exp Med       Date:  2021-02-01       Impact factor: 14.307
  2 in total

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