| Literature DB >> 31649167 |
Aditya S Vaidya1,2, Jonathan D M Helander1,2, Francis C Peterson3, Dezi Elzinga1,2, Wim Dejonghe1,2, Amita Kaundal1,4, Sang-Youl Park1,2, Zenan Xing1,2, Ryousuke Mega5, Jun Takeuchi6,7, Bardia Khanderahoo1,2, Steven Bishay1,2, Brian F Volkman3, Yasushi Todoroki6,7, Masanori Okamoto8,9, Sean R Cutler10,2.
Abstract
Drought causes crop losses worldwide, and its impact is expected to increase as the world warms. This has motivated the development of small-molecule tools for mitigating the effects of drought on agriculture. We show here that current leads are limited by poor bioactivity in wheat, a widely grown staple crop, and in tomato. To address this limitation, we combined virtual screening, x-ray crystallography, and structure-guided design to develop opabactin (OP), an abscisic acid (ABA) mimic with up to an approximately sevenfold increase in receptor affinity relative to ABA and up to 10-fold greater activity in vivo. Studies in Arabidopsis thaliana reveal a role of the type III receptor PYRABACTIN RESISTANCE-LIKE 2 for the antitranspirant efficacy of OP. Thus, virtual screening and structure-guided optimization yielded newly discovered agonists for manipulating crop abiotic stress tolerance and water use.Entities:
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Year: 2019 PMID： 31649167 DOI： 10.1126/science.aaw8848
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728