| Literature DB >> 31647901 |
Masanao Kinoshita1, Kaoru Tanaka2, Nobuaki Matsumori3.
Abstract
The influence of ceramide and its dihydro analog (Cer and DHCer, respectively; inclusively termed Cers) on sphingomyelin (SM) bilayers was examined. Fluorescent microscopy showed that SM/Cers binary bilayers undergo phase separation between Cers-rich and Cers-poor phases. Based on calorimetry, the content of Cers in the Cers-rich phase was estimated and the results show that DHCer in the DHCer-rich phase (17.5 mol%) is more condensed than Cer in the Cer-rich phase (15 mol%), likely due to a stronger intermolecular interaction of DHCer than that of Cer. Furthermore, the Cers-poor phase consists of almost pure SM. X-ray diffraction and water permeability measurements disclosed that the size of crystallites-lipid nano-clusters formed inside the Cers-rich phase-are larger for the DHCer-rich phase than those for the Cer-rich phase. Due to its stronger intermolecular interactions, DHCer could effectively suppress the inter-crystallite packing gaps to avoid the energetic disadvantage, resulting in formation of larger crystallites.Entities:
Keywords: Artificial rafts; Calorimetry; Fluorescent microscopy; Giant unilamellar vesicle; Phase separation; X-ray diffraction
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Year: 2019 PMID： 31647901 DOI： 10.1016/j.chemphyslip.2019.104835
Source DB: PubMed Journal: Chem Phys Lipids ISSN： 0009-3084 Impact factor: 3.329