Literature DB >> 31646506

High-Throughput Fluorescence Assays for Ion Channels and GPCRs.

Irina Vetter1,2, David Carter3, John Bassett4, Jennifer R Deuis3, Bryan Tay3, Sina Jami3, Samuel D Robinson3.   

Abstract

Ca2+, Na+ and K+- permeable ion channels as well as GPCRs linked to Ca2+ release are important drug targets. Accordingly, high-throughput fluorescence plate reader assays have contributed substantially to drug discovery efforts and pharmacological characterization of these receptors and ion channels. This chapter describes some of the basic properties of the fluorescent dyes facilitating these assay approaches as well as general methods for establishment and optimisation of fluorescence assays for ion channels and Gq-coupled GPCRs.

Entities:  

Keywords:  Assay development; FLIPR; Fluorescence imaging; G protein-coupled receptor; High-content; High-throughput; Ligand-gated ion channel; Optimization; Voltage-gated ion channel

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Substances：

Year:  2020        PMID： 31646506     DOI： 10.1007/978-3-030-12457-1_3

Source DB:  PubMed          Journal:  Adv Exp Med Biol        ISSN： 0065-2598            Impact factor:   2.622


  4 in total

1.  A Quantitative Assay for Ca2+ Uptake through Normal and Pathological Hemichannels.

Authors:  Chiara Nardin; Abraham Tettey-Matey; Viola Donati; Daniela Marazziti; Chiara Di Pietro; Chiara Peres; Marcello Raspa; Francesco Zonta; Guang Yang; Maryna Gorelik; Serena Singh; Lia Cardarelli; Sachdev S Sidhu; Fabio Mammano
Journal:  Int J Mol Sci       Date:  2022-06-30       Impact factor: 6.208

2.  Changes in Glial Support of the Hippocampus during the Development of an Alzheimer's Disease-like Pathology and Their Correction by Mitochondria-Targeted Antioxidant SkQ1.

Authors:  Ekaterina A Rudnitskaya; Alena O Burnyasheva; Tatiana A Kozlova; Daniil A Peunov; Nataliya G Kolosova; Natalia A Stefanova
Journal:  Int J Mol Sci       Date:  2022-01-20       Impact factor: 5.923

3.  A New Spectral Shift-Based Method to Characterize Molecular Interactions.

Authors:  Andreas Langer; Tanja Bartoschik; Ondrej Cehlar; Stefan Duhr; Philipp Baaske; Werner Streicher
Journal:  Assay Drug Dev Technol       Date:  2022-02-15       Impact factor: 1.738

Review 4.  Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.

Authors:  Phillip E Klebba; Salete M C Newton; David A Six; Ashish Kumar; Taihao Yang; Brittany L Nairn; Colton Munger; Somnath Chakravorty
Journal:  Chem Rev       Date:  2021-03-16       Impact factor: 60.622
  4 in total

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