| Literature DB >> 31646501 |
Helios Martínez-Banaclocha1, Pablo Pelegrín2.
Abstract
The purinoceptor P2X7 is highly expressed in cells of the innate immune system, including monocytes and macrophages. Its activation is a potent signal to activate the NLRP3 inflammasome and induce the release of proinflammatory cytokines of the IL-1 family. In this chapter, we present a method to monitor NLRP3 inflammasome activation in human monocytes upon P2X7 receptor stimulation by detecting intracellular oligomers of ASC by flow cytometry. This method could be used to evaluate the degree of inflammasome activation in blood samples from patients suffering different chronic inflammatory diseases.Entities:
Keywords: ASC; ATP; Flow cytometry; Inflammasome; Monocyte; NLRP3; P2X7
Mesh：
Substances：
Year: 2020 PMID： 31646501 DOI： 10.1007/978-1-4939-9717-6_25
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745