| Literature DB >> 31644995 |
Rosalba Monica Ferraro1, Gaetana Lanzi2, Stefania Masneri2, Chiara Barisani2, Giovanna Piovani3, Giulia Savio3, Marco Cattalini4, Jessica Galli5, Cristina Cereda6, Marco Muzi-Falconi7, Simona Orcesi8, Elisa Fazzi5, Silvia Giliani2.
Abstract
Fibroblasts from a patient with Aicardi Goutières Syndrome (AGS) carrying a compound heterozygous mutation in TREX1, were reprogrammed into induced pluripotent stem cells (iPSCs) to establish isogenic clonal stem cell lines: UNIBSi006-A, UNIBSi006-B, and UNIBSi006-C. Cells were transduced using the episomal Sendai viral vectors, containing human OCT4, SOX2, c-MYC and KLF4 transcription factors. The transgene-free iPSC lines showed normal karyotype, expressed pluripotent markers and displayed in vitro differentiation potential toward cells of the three embryonic germ layers.Entities:
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Year: 2019 PMID： 31644995 DOI： 10.1016/j.scr.2019.101580
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020