Ting Lu1, Yingchun Zheng2, Haiying Yang3, Buling Wu1, Jun Xiong4, Cheng Huang2, Yuhua Pan1, Meiyi Li2, Fei He2, Fu Xiong2. 1. School of Stomatology, Southern Medical University; Department of Endodontics, National Key Specialty of Endodontics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. 2. School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China. 3. Department of Laboratory Animal Center, Southern Medical University, Guangzhou 510515, China. 4. Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Abstract
OBJECTIVE: To explore the histological structure of the deciduous teeth and the tooth germs of Tibetan miniature pigs for studies of dental tissue diseases and tooth regeneration. METHODS: The structure of the deciduous teeth of Tibetan miniature pigs was observed by X-ray. The ultrastructure of the enamel and dentin of deciduous teeth was characterized by scanning electron microscopy. The jaws and teeth were three-dimensionally reconstructed using Mimics software based on Micro-CT scanning of the deciduous teeth. Image J software was used to calculate the gray value and the mineralization density of the deciduous teeth. Hisotological structure of the tooth germ and the pulp tissue of Tibetan miniature pigs was observed using HE staining. RESULTS: The deciduous teeth of Tibetan miniature pigs were composed of enamel, dentin and medullary pulp tissue. The permanent tooth germ were formed during the deciduous dentition. The enamel and dentin ultrastructure of deciduous teeth were consistent with that of human deciduous teeth. The enamel and dentin mineralization densities were 2.47±0.09 g/cm3 and 1.72±0.07 g/cm3, respectively. The pathological structures of tooth germ and pulp tissue were similar to those of human teeth, and the pulp tissue of the deciduous teeth was in an undifferentiated state. CONCLUSIONS: The deciduous teeth of Tibetan miniature pig have similar anatomy, ultrastructure and histopathological structure to human teeth and can serve as a good animal model for studying human dental tissue diseases and the mechanisms of tooth regeneration.
OBJECTIVE: To explore the histological structure of the deciduous teeth and the tooth germs of Tibetan miniature pigs for studies of dental tissue diseases and tooth regeneration. METHODS: The structure of the deciduous teeth of Tibetan miniature pigs was observed by X-ray. The ultrastructure of the enamel and dentin of deciduous teeth was characterized by scanning electron microscopy. The jaws and teeth were three-dimensionally reconstructed using Mimics software based on Micro-CT scanning of the deciduous teeth. Image J software was used to calculate the gray value and the mineralization density of the deciduous teeth. Hisotological structure of the tooth germ and the pulp tissue of Tibetan miniature pigs was observed using HE staining. RESULTS: The deciduous teeth of Tibetan miniature pigs were composed of enamel, dentin and medullary pulp tissue. The permanent tooth germ were formed during the deciduous dentition. The enamel and dentin ultrastructure of deciduous teeth were consistent with that of human deciduous teeth. The enamel and dentin mineralization densities were 2.47±0.09 g/cm3 and 1.72±0.07 g/cm3, respectively. The pathological structures of tooth germ and pulp tissue were similar to those of human teeth, and the pulp tissue of the deciduous teeth was in an undifferentiated state. CONCLUSIONS: The deciduous teeth of Tibetan miniature pig have similar anatomy, ultrastructure and histopathological structure to human teeth and can serve as a good animal model for studying human dental tissue diseases and the mechanisms of tooth regeneration.
Authors: Monika Baxa; Marian Hruska-Plochan; Stefan Juhas; Petr Vodicka; Antonin Pavlok; Jana Juhasova; Atsushi Miyanohara; Tetsuya Nejime; Jiri Klima; Monika Macakova; Silvia Marsala; Andreas Weiss; Svatava Kubickova; Petra Musilova; Radek Vrtel; Emily M Sontag; Leslie M Thompson; Jan Schier; Hana Hansikova; David S Howland; Elena Cattaneo; Marian DiFiglia; Martin Marsala; Jan Motlik Journal: J Huntingtons Dis Date: 2013
Authors: Wennan Li; Xingjuan Chen; Ashley M Riley; S Christopher Hiett; Constance J Temm; Eleni Beli; Xin Long; Saikat Chakraborty; Mouhamad Alloosh; Fletcher A White; Maria B Grant; Michael Sturek; Alexander G Obukhov Journal: Basic Res Cardiol Date: 2017-07-29 Impact factor: 17.165