Linoj Samuel1. 1. Department of Pathology and Laboratory Medicine, Clinical Microbiology Division, Henry Ford Health System, Detroit, MI. lsamuel2@hfhs.org.
Abstract
BACKGROUND: Advances in medicine have improved our understanding of sepsis, but it remains a major cause of morbidity and mortality. The detection of pathogens that cause sepsis remains a challenge for clinical microbiology laboratories. CONTENT: Routine blood cultures are time-consuming and are negative in a large proportion of cases, leading to excessive use of broad-spectrum antimicrobials. Molecular testing direct from patient blood without the need for incubation has the potential to fill the gaps in our diagnostic armament and complement blood cultures to provide results in a timely manner. Currently available platforms show promise but have yet to definitively address gaps in sensitivity and specificity. SUMMARY: Significant strides have been made in the detection of pathogens directly from blood. A number of hurdles, however, remain before this technology can be adapted for routine use.
BACKGROUND: Advances in medicine have improved our understanding of sepsis, but it remains a major cause of morbidity and mortality. The detection of pathogens that cause sepsis remains a challenge for clinical microbiology laboratories. CONTENT: Routine blood cultures are time-consuming and are negative in a large proportion of cases, leading to excessive use of broad-spectrum antimicrobials. Molecular testing direct from patient blood without the need for incubation has the potential to fill the gaps in our diagnostic armament and complement blood cultures to provide results in a timely manner. Currently available platforms show promise but have yet to definitively address gaps in sensitivity and specificity. SUMMARY: Significant strides have been made in the detection of pathogens directly from blood. A number of hurdles, however, remain before this technology can be adapted for routine use.
Authors: Ryan J McGuire; Sean C Yu; Philip R O Payne; Albert M Lai; M Cristina Vazquez-Guillamet; Marin H Kollef; Andrew P Michelson Journal: Antimicrob Agents Chemother Date: 2021-06-17 Impact factor: 5.191
Authors: Omary Mzava; Alexandre Pellan Cheng; Adrienne Chang; Sami Smalling; Liz-Audrey Kounatse Djomnang; Joan Sesing Lenz; Randy Longman; Amy Steadman; Luis G Gómez-Escobar; Edward J Schenck; Mirella Salvatore; Michael J Satlin; Manikkam Suthanthiran; John R Lee; Christopher E Mason; Darshana Dadhania; Iwijn De Vlaminck Journal: Nat Commun Date: 2022-07-21 Impact factor: 17.694