| Literature DB >> 31634414 |
Xiaoping Bao1,2,3, Maroof M Adil1,2, Riya Muckom1,2, Joshua A Zimmermann1,2, Aurelie Tran4, Natalie Suhy4, Yibo Xu3, Rocío G Sampayo1,2, Douglas S Clark2,5, David V Schaffer1,2,3,4.
Abstract
Transcription factors (TFs) are potent proteins that control gene expression and can thereby drive cell fate decisions. Fluorescent reporters have been broadly knocked into endogenous TF loci to investigate the biological roles of these factors; however, the sensitivity of such analyses in human pluripotent stem cells (hPSCs) is often compromised by low TF expression levels and/or reporter silencing. Complementarily, we report an inducible and quantitative reporter platform based on the Cre-LoxP recombination system that enables robust, quantifiable, and continuous monitoring of live hPSCs and their progeny to investigate the roles of TFs during human development and disease. Stem Cells 2019;37:1556-1566. ©AlphaMed Press 2019.Entities:
Keywords: CRISPR/Cas9; Fate mapping; Gene editing; Human pluripotent stem cells; Transcription factors
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Year: 2019 PMID： 31634414 DOI： 10.1002/stem.3096
Source DB: PubMed Journal: Stem Cells ISSN： 1066-5099 Impact factor: 6.277