Xianzhen Feng1, Jin Zhao2, Jingjing Ding1, Xiaoyi Shen1, Jun Zhou1, Zhongqing Xu1. 1. Department of General Practice, Tongren Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200336, China. 2. Department of General Practice, Shanghai Pudong New Area People's Hospital Shanghai 201299, China.
Abstract
BACKGROUND AND OBJECTIVES: Diabetic nephropathy (DN) is one of the commonest microvascular complications of diabetes and has been the major cause of end-stage renal disease in many countries. It is of great clinical significance to further explore more efficacious therapeutic strategies for DN. This study aims to explore the effect of Blnc1 on renal fibrosis in diabetic nephropathy. METHODS: In this study, mRNA level of Blnc1 was examined by RT-PCR. HE staining and Masson staining were adopted to detect kidney damage and renal fibrosis. The renal fibrosis was evaluated by the levels of PTEN, fibronectin, collagen I and collagen IV with immunofluorescence assay and western blot analysis. Oxidative Stress and inflammatory response were detected by ELISA assay. At the same time, western blot was performed to detect the proteins related to NRF2/HO-1 and NF-κB pathways. RESULTS: Blnc1 has higher expression in serum of DN patients, STZ-induced DN model and HG-induced HK2 cells. Blnc1 interference significantly attenuated renal fibrosis, inflammation and oxidative stress via NRF2/HO-1 and NF-κB pathways. CONCLUSION: Our present study suggested that Blnc1 can affect inflammation, oxidative stress and renal fibrosis by Nrf2/HO-1 and NF-κB pathways in DN. AJTR
BACKGROUND AND OBJECTIVES:Diabetic nephropathy (DN) is one of the commonest microvascular complications of diabetes and has been the major cause of end-stage renal disease in many countries. It is of great clinical significance to further explore more efficacious therapeutic strategies for DN. This study aims to explore the effect of Blnc1 on renal fibrosis in diabetic nephropathy. METHODS: In this study, mRNA level of Blnc1 was examined by RT-PCR. HE staining and Masson staining were adopted to detect kidney damage and renal fibrosis. The renal fibrosis was evaluated by the levels of PTEN, fibronectin, collagen I and collagen IV with immunofluorescence assay and western blot analysis. Oxidative Stress and inflammatory response were detected by ELISA assay. At the same time, western blot was performed to detect the proteins related to NRF2/HO-1 and NF-κB pathways. RESULTS: Blnc1 has higher expression in serum of DNpatients, STZ-induced DN model and HG-induced HK2 cells. Blnc1 interference significantly attenuated renal fibrosis, inflammation and oxidative stress via NRF2/HO-1 and NF-κB pathways. CONCLUSION: Our present study suggested that Blnc1 can affect inflammation, oxidative stress and renal fibrosis by Nrf2/HO-1 and NF-κB pathways in DN. AJTR
Authors: Rongpei Lan; Hui Geng; Aaron J Polichnowski; Prajjal K Singha; Pothana Saikumar; Donald G McEwen; Karen A Griffin; Robert Koesters; Joel M Weinberg; Anil K Bidani; Wilhelm Kriz; Manjeri A Venkatachalam Journal: Am J Physiol Renal Physiol Date: 2012-02-01
Authors: Xu-Yun Zhao; Siming Li; Jennifer L DelProposto; Tongyu Liu; Lin Mi; Cara Porsche; Xiaoling Peng; Carey N Lumeng; Jiandie D Lin Journal: Mol Metab Date: 2018-06-08 Impact factor: 7.422