| Literature DB >> 3163238 |
J L Cone1, D R Brown, J E DeLarco.
Abstract
Platelets contain high concentrations of TGF-beta and for this reason have been the primary source of materials for its purification. The yield from acid-ethanol extracts of platelets has been reported to be 0.5 microgram/unit of platelets. We have developed a method which yields approximately 2 micrograms/unit of platelets from acid-ethanol extracts. The use of an ion-exchange column followed by RPLC results in a homogeneous product in less time and with fewer manipulations than the previously published methods. A tritiated thymidine incorporation assay using the CC164 cell line is described for the quantitation of TGF-beta.Entities:
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Year: 1988 PMID： 3163238 DOI： 10.1016/0003-2697(88)90011-5
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365