Shafigheh Asgari-Karchekani1, Mohammad Karimian1,2, Tahereh Mazoochi1,2, Maryam Akhavan Taheri1, Tahereh Khamehchian3,4. 1. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran. 2. Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran. 3. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran. taherehkhamehchian@gmail.com. 4. Department of Pathology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran. taherehkhamehchian@gmail.com.
Abstract
PURPOSE: Caudal-type homeobox transcription factor 2 (CDX2) is expressed in the nucleus of the intestinal epithelial cells and is essential for embryonic formation and differentiation of the intestine, whose reduced expression can result in metastatic tumors. This study was to investigate the association of CDX2 expression level in colorectal cancer (CRC) with age, gender, microscopic histopathology, tumor staging, tumor grading, 3-year survival rate, and prognosis. METHODS: After preparing paraffin tissue blocks, CDX2 protein expression was assayed by immunohistochemistry in 82 CRC patients. Hematoxylin and eosin staining was used to detect tumor histology, tumor grading, tumor staging, and blood-lymphatic, and neural invasion. The collected data includes age, gender, tumor site, and 3-year survival rate of patients after diagnosis. RESULTS: The CDX2 expression was significantly higher in men than in women, and it was significantly lower in right-sided tumors as in transverse colon and left-sided tumors. Also, the CDX2 expression was significantly higher in adenocarcinoma than in mucinous. In addition, a significant correlation was found between downregulated CDX2 and lymph node involvement. In tumor grading, there was a significant correlation between CDX2 downregulation and high-grade tumor. Moreover, there was a significant correlation between downregulated CDX2 expression and overall pathological staging. CONCLUSION: The downregulated CDX2 expression is associated with female gender, right-sided tumors, mucinous tumors, lymph node involvement, high-grade tumor, and advanced overall pathological staging and can be considered as a possible prognostic factor for patients follow-up. However, our study is a preliminary study and further studies with larger sample sizes in different ethnic groups are required.
PURPOSE:Caudal-type homeobox transcription factor 2 (CDX2) is expressed in the nucleus of the intestinal epithelial cells and is essential for embryonic formation and differentiation of the intestine, whose reduced expression can result in metastatic tumors. This study was to investigate the association of CDX2 expression level in colorectal cancer (CRC) with age, gender, microscopic histopathology, tumor staging, tumor grading, 3-year survival rate, and prognosis. METHODS: After preparing paraffin tissue blocks, CDX2 protein expression was assayed by immunohistochemistry in 82 CRCpatients. Hematoxylin and eosin staining was used to detect tumor histology, tumor grading, tumor staging, and blood-lymphatic, and neural invasion. The collected data includes age, gender, tumor site, and 3-year survival rate of patients after diagnosis. RESULTS: The CDX2 expression was significantly higher in men than in women, and it was significantly lower in right-sided tumors as in transverse colon and left-sided tumors. Also, the CDX2 expression was significantly higher in adenocarcinoma than in mucinous. In addition, a significant correlation was found between downregulated CDX2 and lymph node involvement. In tumor grading, there was a significant correlation between CDX2 downregulation and high-grade tumor. Moreover, there was a significant correlation between downregulated CDX2 expression and overall pathological staging. CONCLUSION: The downregulated CDX2 expression is associated with female gender, right-sided tumors, mucinous tumors, lymph node involvement, high-grade tumor, and advanced overall pathological staging and can be considered as a possible prognostic factor for patients follow-up. However, our study is a preliminary study and further studies with larger sample sizes in different ethnic groups are required.
Authors: Shu Wang; Yi Jia Ma; Yong Shi Li; Xu Sheng Ge; Chang Lu; Chun Bo Cai; Yang Yang; Yan Zhao; Guo Ming Liang; Xiao Hong Guo; Guo Qing Cao; Bu Gao Li; Peng Fei Gao Journal: Anim Biosci Date: 2022-03-02
Authors: Francesca Pia Caruso; Mario Rosario D'Andrea; Luigi Coppola; Matteo Landriscina; Valentina Condelli; Luigi Cerulo; Guido Giordano; Almudena Porras; Massimo Pancione Journal: Cancer Cell Int Date: 2022-08-11 Impact factor: 6.429