Kun Wang1,2, Xiao Long Li3, Jinbo Liu2, Xiaoliang Sun2, Huilin Yang4, Xin Gao5. 1. Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. 2. Department of Orthopedics, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China. 3. Department of Orthopedics, The People's Hospital of Wujin Affiliated with Jiangsu University, Changzhou, 213017, China. 4. Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. 616841263@qq.com. 5. Department of Orthopedics, The People's Hospital of Wujin Affiliated with Jiangsu University, Changzhou, 213017, China. xgao01@163.com.
Abstract
PURPOSE: Hyaluronic acid prevents tissue adhesion after different surgeries. Physical barriers and inflammatory regulation have been suggested to be involved in the mechanism of these clinical effects. However, the molecular mechanism by which hyaluronic acid prevents epidural adhesion has not yet been reported. METHODS: In the current in vivo studies, we investigated cross-linked hyaluronic acid gel in the regulation of scar gene expression, the accumulation of fibroblasts in scar tissue, and the prevention of epidural adhesion. The effect of cross-linked hyaluronic acid gel on the secretion of inflammatory factors was observed in vitro. In addition, to ensure the accuracy and reliability of the in vivo gene expression results, we used a cell model to detect the target genes in vitro. RESULTS: The expression levels of TGFβ1 and COL1A1 mRNA were decreased in the cross-linked hyaluronic acid gel-treated group, and the protein expression of levels TGFβ1 and COL1A1 were also reduced, as detected by Western blotting in vitro and in vivo (P < 0.05). Histomorphometry results demonstrated that the number of fibroblasts in the experimental group was significantly lower than that in the control group 2 weeks postoperatively. Micro-CT scans showed that the cross-linked hyaluronic acid gel could reduce adhesion in the epidural space after laminectomy. Additionally, the cross-linked hyaluronic acid gel could inhibit IL-6 secretion. CONCLUSIONS: These results indicate that cross-linked hyaluronic acid gel can prevent epidural adhesion by inhibiting inflammatory factors, such as IL-6, and downregulating TGFβ1 and COL1A1 mRNA expression. These slides can be retrieved under Electronic Supplementary Material.
PURPOSE:Hyaluronic acid prevents tissue adhesion after different surgeries. Physical barriers and inflammatory regulation have been suggested to be involved in the mechanism of these clinical effects. However, the molecular mechanism by which hyaluronic acid prevents epidural adhesion has not yet been reported. METHODS: In the current in vivo studies, we investigated cross-linked hyaluronic acid gel in the regulation of scar gene expression, the accumulation of fibroblasts in scar tissue, and the prevention of epidural adhesion. The effect of cross-linked hyaluronic acid gel on the secretion of inflammatory factors was observed in vitro. In addition, to ensure the accuracy and reliability of the in vivo gene expression results, we used a cell model to detect the target genes in vitro. RESULTS: The expression levels of TGFβ1 and COL1A1 mRNA were decreased in the cross-linked hyaluronic acid gel-treated group, and the protein expression of levels TGFβ1 and COL1A1 were also reduced, as detected by Western blotting in vitro and in vivo (P < 0.05). Histomorphometry results demonstrated that the number of fibroblasts in the experimental group was significantly lower than that in the control group 2 weeks postoperatively. Micro-CT scans showed that the cross-linked hyaluronic acid gel could reduce adhesion in the epidural space after laminectomy. Additionally, the cross-linked hyaluronic acid gel could inhibit IL-6 secretion. CONCLUSIONS: These results indicate that cross-linked hyaluronic acid gel can prevent epidural adhesion by inhibiting inflammatory factors, such as IL-6, and downregulating TGFβ1 and COL1A1 mRNA expression. These slides can be retrieved under Electronic Supplementary Material.
Authors: Aimee L Schimizzi; Jennifer B Massie; Mark Murphy; Andrew Perry; Choll W Kim; Steven R Garfin; Wayne H Akeson Journal: Spine J Date: 2006-07-24 Impact factor: 4.166
Authors: N de Tribolet; F Porchet; T W Lutz; O Gratzl; J Brotchi; H A van Alphen; R E van Acker; A Benini; K N Strommer; R L Bernays; J Goffin; E A Beuls; J S Ross Journal: Am J Orthop (Belle Mead NJ) Date: 1998-02
Authors: A A Bakibaev; V P Tuguldurova; M V Lyapunova; V V Ivanov; O A Kaidash; E V Udut; M V Bukterov; E E Buyko; A S Kasyanova; V S Malkov Journal: Sovrem Tekhnologii Med Date: 2021-02-28