Microbial lipid production from dilute acid and dilute alkali pretreated corn stover via Trichosporon dermatis.

Microbial lipid production from lignocellulosic biomass has attracted much attention recently. In this study, T. dermatis 32903 was selected from eleven promising oleaginous yeast strains. Carbon to nitrogen ratio (C/N) was investigated and optimized to maximize lipid production. Dilute acid (DA) pretreated corn stover (CS) and dilute alkali (AL) pretreated CS were then used for microbial lipid production, resulting in lipid concentrations of 7.46 g/L and 6.81 g/L, with sugar to lipid yields reached 0.104 g/g and 0.101 g/g, respectively. Washing of DA-CS and AL-CS enhanced lipid production to 11.43 g/L and 20.36 g/L with sugar to lipid yields improved to 0.156 g/g and 0.186 g/g, respectively. As degradation products in pretreated biomass showed severe inhibition on lipid fermentation, eight typical degradation products were further investigated for their effects on lipid fermentation. T. dermatis 32903 exhibited high tolerance to furan derivatives and week acids, but lower tolerance to phenolic compounds.