Literature DB >> 3163

Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland.

S A Betts, R J Mayer.   

Abstract

1. 6-Phosphogluconate dehydrogenase from rabbit mammary gland was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the subunit is 52 000. The enzyme was purified 150-fold with a final specific activity of 20 mumol of NADP+ reduced/min per mg of protein and overall yield of 3%. The molecular weight of the native enzyme is estimated to be 104 000 from gel-filtration studies. The final purification step was carried out by affinity chromatography with NADP+-Sepharose. 2. The Km values for 6-phosphogluconate and NADP+ are approx. 54 muM and 23 muM respectively. 3. Citrate and pyrophosphate are competitive inhibitors of the enzyme with respect to both 6-phosphogluconate and NADP+. 4. MgCl2 affects the apparent Km for NADP+ at saturating concentrations of 6-phosphogluconate.

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Year:  1975        PMID： 3163      PMCID： PMC1172355          DOI： 10.1042/bj1510263

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver.

Authors:  G E GLOCK; P McLEAN
Journal:  Biochem J       Date:  1953-10       Impact factor: 3.857

2.  Crystalline D-gluconate 6-phosphate dehydrogenase.

Authors:  S PONTREMOLI; A DE FLORA; E GRAZI; A BONSIGNORE; B L HORECKER
Journal:  J Biol Chem       Date:  1961-11       Impact factor: 5.157

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Sheep liver 6-phosphogluconate dehydrogenase. Inhibition by nucleoside phosphates and by other metabolic intermediates.

Authors:  J E Dyson; R E D'Orazio
Journal:  J Biol Chem       Date:  1973-08-10       Impact factor: 5.157

5.  Purification and properties of rat liver 6-phosphogluconate dehydrogenase. Activity at normal in vivo concentration of coenzyme.

Authors:  D Procsal; D Holten
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

6.  Studies on the fractionation of mucosal homogenates from the small intestine.

Authors:  G Hübscher; G R West; D N Brindley
Journal:  Biochem J       Date:  1965-12       Impact factor: 3.857

7.  Covalent coupling of nucleotides to agarose for affinity chromatography.

Authors:  R Lamed; Y Levin; M Wilchek
Journal:  Biochim Biophys Acta       Date:  1973-04-28

8.  Human 6-phosphogluconate dehydrogenase. Purification of the erythrocyte enzyme and the influence of ions and NADPH on its activity.

Authors:  B M Pearse; M A Rosemeyer
Journal:  Eur J Biochem       Date:  1974-02-15

9.  Enzymic changes in rabbit and rat mammary gland during the lactation cycle.

Authors:  B Gul; R Dils
Journal:  Biochem J       Date:  1969-04       Impact factor: 3.857

10.  Influence of hormones on liver. I. Effects of steroids and thyroxine on pyridine nucleotide-linked dehydrogenases.

Authors:  C HUGGINS; F O YAO
Journal:  J Exp Med       Date:  1959-12-01       Impact factor: 14.307
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  9 in total

1.  Absorption of antisera for studies on specific enzyme turnover.

Authors:  J H Walker; S A Betts; R Manning; R J Mayer
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

2.  Molecular weight and subunit size of fatty acid synthetase from rabbit mammary gland.

Authors:  N Paskin; R J Mayer
Journal:  Biochem J       Date:  1976-10-01       Impact factor: 3.857

3.  Purification and some properties of acetyl-coenzyme A carboxylase from rabbit mammary gland.

Authors:  R Manning; R Dils; R J Mayer
Journal:  Biochem J       Date:  1976-02-01       Impact factor: 3.857

4.  6-Phosphogluconate Dehydrogenase Isoenzymes from the Developing Endosperm of Ricinus communis L.

Authors:  P D Simcox; D T Dennis
Journal:  Plant Physiol       Date:  1978-08       Impact factor: 8.340

5.  Purification and kinetic properties of 6-phosphogluconate dehydrogenase from rat small intestine.

Authors:  Deniz Ceyhan; Ali Danişan; I Hamdi Oğüş; Nazmi Ozer
Journal:  Protein J       Date:  2005-07       Impact factor: 2.371

6.  An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase.

Authors:  P Griffiths; A Houlton; M J Adams; M J Harvey; P D Dean
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

7.  The formation of lipid-linked sugars by cell-free preparations of lactating rabbit mammary gland.

Authors:  D A White
Journal:  Biochem J       Date:  1978-03-15       Impact factor: 3.857

8.  6-Phosphogluconate dehydrogenase from Drosophila melanogaster. I. Purification and properties of the A isozyme.

Authors:  J H Williamson; D Krochko; B W Geer
Journal:  Biochem Genet       Date:  1980-02       Impact factor: 1.890

9.  Purification and immunochemical characterization of monoamine oxidase from rat and human liver.

Authors:  R G Dennick; R J Mayer
Journal:  Biochem J       Date:  1977-01-01       Impact factor: 3.857
  9 in total

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