| Literature DB >> 3162810 |
M Gigli1, S M Doglia, J M Millot, L Valentini, M Manfait.
Abstract
Doxorubicin-DNA association has been studied by quantitative microspectrofluorometry. Fluorescence emission spectra from a microvolume of single living cell nuclei treated with doxorubicin have been analyzed in terms of difference in spectral shape and fluorescence yield between free and DNA-bound drug. Contribution of each spectral component to the total signal was calculated by least-squares linear regression. With this method of analysis, total drug concentration has been determined with an error of less than 10%. Moreover, the uptake into the nucleus has been studied in a non destructive way, avoiding use of 14C-labelled drug. Kinetic studies of drug accumulation into the nuclei were conducted on sensitive and resistant cells.Entities:
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Year: 1988 PMID： 3162810 DOI： 10.1016/0167-4781(88)90068-1
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002