Incorporation of bioflavonoids from Bidens tripartite into micelles of non-ionic surfactants - experimental and theoretical studies.

Classical extraction methods used for an isolation of active substances from the plant material are expensive, complicated and often environmentally unfriendly. The ultrasonic assistance micelle-mediated extraction method (UAMME) seems to be an interesting alternative. The aim of this work was to compare an efficiency of water solutions of three non-ionic surfactants: C9-11 Pareth-5, PPG-6 Steareth-7 and PPG-4 Laureth-5 in UAMME of Bidens tripartita. Subsequently, the obtained extracts were separated into two immiscible phases, a polyphenols rich surfactants phase and an aqueous phase by its heating above surfactants cloud points (CPC) or by salting out with NaCl. Along with decreasing the Hydrophilic/Lipophilic Balance (HLB) factor value of surfactants, i.e. increase of the hydrophobicity, a significant decreasing of the flavonoid content was observed. While polyphenols content and antioxidant activity increased. The good surface properties of all surfactants correspond to the high content of phenolic compounds in extracts and both concentration methods resulted in even a 50-fold increase of polyphenols content. Dynamic light scattering measurements (DLS) provided that solubilization of polyphenols, i.e. their incorporation into surfactants' micelles, occurred with significant enlarging of particle size. Based on the molecular dynamic simulations, the mechanism of polyphenols incorporation into micelles was discussed.