Literature DB >> 31625095

Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning.

Kathryn M Frietze1,2, Susan B Core3, Alexandria Linville3, Bryce Chackerian3, David S Peabody3.   

Abstract

Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations. Here, we describe a method for using a phage-display platform to assess antibody specificity in human serum. We describe the method with reference to the bacteriophage MS2 virus-like particle (VLP) platform, but it can be adapted to other phage-display technologies as well.

Entities:  

Keywords:  Affinity selection; Antibodies; Deep sequencing; Pathogens; Phage display; Serum; Virus-like particle

Mesh：

Substances：

Year:  2020        PMID： 31625095     DOI： 10.1007/978-1-4939-9853-1_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Epitope-Based Vaccines against the Chlamydia trachomatis Major Outer Membrane Protein Variable Domain 4 Elicit Protection in Mice.

Authors:  Amanda L Collar; Alexandria C Linville; Susan B Core; Kathryn M Frietze
Journal:  Vaccines (Basel)       Date:  2022-05-30

2.  Expansion and Refinement of Deep Sequence-Coupled Biopanning Technology for Epitope-Specific Antibody Responses in Human Serum.

Authors:  Nikole L Warner; Alexandria C Linville; Susan B Core; Brechla Moreno; Juan M Pascale; David S Peabody; Bryce Chackerian; Kathryn M Frietze
Journal:  Viruses       Date:  2020-09-30       Impact factor: 5.048

3.  Engineering an Antibody V Gene-Selective Vaccine.

Authors:  Larance Ronsard; Ashraf S Yousif; Julianne Peabody; Vintus Okonkwo; Pascal Devant; Alemu Tekewe Mogus; Ralston M Barnes; Daniel Rohrer; Nils Lonberg; David Peabody; Bryce Chackerian; Daniel Lingwood
Journal:  Front Immunol       Date:  2021-09-09       Impact factor: 7.561
  3 in total

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