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Literature DB >> 3162159

A convenient technique to compare the efficiency of promoters in Escherichia coli.

Abstract

We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.

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Year: 1985 PMID： 3162159 PMCID： PMC321922 DOI： 10.1093/nar/13.16.5919

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Essential and nonessential sequences in malPp, a positively controlled promoter in Escherichia coli.

Authors: O Raibaud; C Gutierrez; M Schwartz
Journal: J Bacteriol Date: 1985-03 Impact factor: 3.490

2. Use of transcriptional repressors to stabilize plasmid copy number of transcriptional fusion vectors.

Authors: P F Lambert; W S Reznikoff
Journal: J Bacteriol Date: 1985-04 Impact factor: 3.490

3. The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.

Authors: S Froshauer; J Beckwith
Journal: J Biol Chem Date: 1984-09-10 Impact factor: 5.157

4. Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter.

Authors: T P Malan; A Kolb; H Buc; W R McClure
Journal: J Mol Biol Date: 1984-12-25 Impact factor: 5.469

5. Evidence that the 5' end of lac mRNA starts to decay as soon as it is synthesized.

Authors: V J Cannistraro; D Kennell
Journal: J Bacteriol Date: 1985-02 Impact factor: 3.490

6. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe.

Authors: C Yanofsky; R L Kelley; V Horn
Journal: J Bacteriol Date: 1984-06 Impact factor: 3.490

7. Role of DNA regions flanking the tryptophan promoter of Escherichia coli. I. Insertion of synthetic oligonucleotides.

Authors: D R Russell; E A Auger; P S Vermersch; G N Bennett
Journal: Gene Date: 1984-12 Impact factor: 3.688

8. A technique for integrating any DNA fragment into the chromosome of Escherichia coli.

Authors: O Raibaud; M Mock; M Schwartz
Journal: Gene Date: 1984 Jul-Aug Impact factor: 3.688

9. The mac promoters: functional hybrid promoters activated by the malT product and repressed by the lacI product.

Authors: D Vidal-Ingigliardi; O Raibaud
Journal: Nucleic Acids Res Date: 1985-02-25 Impact factor: 16.971

10. Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

Authors: D Stueber; H Bujard
Journal: EMBO J Date: 1982 Impact factor: 11.598

24 in total

1. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli.

Authors: M Davalos-Garcia; A Conter; I Toesca; C Gutierrez; K Cam
Journal: J Bacteriol Date: 2001-10 Impact factor: 3.490

2. Interactions between the 2.4 and 4.2 regions of sigmaS, the stress-specific sigma factor of Escherichia coli, and the -10 and -35 promoter elements.

Authors: Claire Checroun; Patricia Bordes; Olivier Leroy; Annie Kolb; Claude Gutierrez
Journal: Nucleic Acids Res Date: 2004-01-02 Impact factor: 16.971

3. Transcription of single-copy hybrid lacZ genes by T7 RNA polymerase in Escherichia coli: mRNA synthesis and degradation can be uncoupled from translation.

Authors: M Chevrier-Miller; N Jacques; O Raibaud; M Dreyfus
Journal: Nucleic Acids Res Date: 1990-10-11 Impact factor: 16.971

A convenient technique to compare the efficiency of promoters in Escherichia coli.

1. Essential and nonessential sequences in malPp, a positively controlled promoter in Escherichia coli.

2. Use of transcriptional repressors to stabilize plasmid copy number of transcriptional fusion vectors.

3. The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.

4. Mechanism of CRP-cAMP activation of lac operon transcription initiation activation of the P1 promoter.

5. Evidence that the 5' end of lac mRNA starts to decay as soon as it is synthesized.

6. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe.

7. Role of DNA regions flanking the tryptophan promoter of Escherichia coli. I. Insertion of synthetic oligonucleotides.

8. A technique for integrating any DNA fragment into the chromosome of Escherichia coli.

9. The mac promoters: functional hybrid promoters activated by the malT product and repressed by the lacI product.

10. Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

1. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli.

2. Interactions between the 2.4 and 4.2 regions of sigmaS, the stress-specific sigma factor of Escherichia coli, and the -10 and -35 promoter elements.

3. Transcription of single-copy hybrid lacZ genes by T7 RNA polymerase in Escherichia coli: mRNA synthesis and degradation can be uncoupled from translation.

4. Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion.

5. Lon-dependent regulation of the DNA binding protein HU in Escherichia coli.

6. Cloning, characterization, and expression of the dapE gene of Escherichia coli.

7. A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome.

8. Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction.

9. An Escherichia coli host strain useful for efficient overproduction of cloned gene products with NaCl as the inducer.

10. Analysis of E. coli promoter sequences.