Nan Sun1, Rongguang Zhang2, Guangcai Duan1, Xiaoyan Peng1, Chen Wang1, Shuaiyin Chen1, Qingtang Fan1. 1. Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, China. 2. Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, China. zrg@zzu.edu.cn.
Abstract
OBJECTIVE: To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. pylori oral vaccines and functional foods. RESULTS: Lpp20 was expressed as a 22 kDa protein in Lactococcus lactis, constituting 11.2% of the cell lysate proteins, and recognized by mouse antisera. Mice orally gavaged with the engineered bacterium had elevated serum IgG levels and lowered urease activity of stomach following H. pylori challenges. CONCLUSIONS: This study firstly reports a food-grade L. lactis strain delivering Lpp20 to mucosal immunization sites, demonstrating a novel efficient production and safe utilization mode of Lpp20, offering a promising vaccine candidate and health food sources.
OBJECTIVE: To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. pylori oral vaccines and functional foods. RESULTS: Lpp20 was expressed as a 22 kDa protein in Lactococcus lactis, constituting 11.2% of the cell lysate proteins, and recognized by mouse antisera. Mice orally gavaged with the engineered bacterium had elevated serum IgG levels and lowered urease activity of stomach following H. pylori challenges. CONCLUSIONS: This study firstly reports a food-grade L. lactis strain delivering Lpp20 to mucosal immunization sites, demonstrating a novel efficient production and safe utilization mode of Lpp20, offering a promising vaccine candidate and health food sources.