Maryam Mozafari1, Arezoo Khoradmehr2, Amirhossein Danafar3, Mohsen Miresmaeili4, Seyed Mehdi Kalantar2. 1. Department of Biology, Ashkezar Islamic Azad University, Yazd, Iran. 2. Research and Clinical Center for Infertility, Yazd Reproduction Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 3. Seyedhassani Medical Sciences Center, Yazd, Iran. 4. Science and Arts University, Biology department, Yazd, Iran.
Abstract
BACKGROUND: Silver nanoparticles (SNPs) are being increasingly used in medical and industrial products. The aim of the present study was to evaluate the toxic effect of maternal exposure to SNP (1 mg/kg/day, 70 nm) on fetal development during the first and second weeks of pregnancy. METHODS: Twenty-four pregnant mice were divided into four groups. SNP was administered by oral gavage on gestational days (GD) 1-7, GD8-14, or GD1-14. Phosphate buffered saline was administered by oral gavage to a control group. On GD15, the uteri were excised, and fetal bodies and placentas were weighed. Head and placental circumferences and fetal crown-rump length were measured, and fetuses were evaluated for external malformation. TUNEL assay was performed to assess apoptosis in the fetal midbrain. Hematoxylin-eosin staining was carried out to determine changes in fetal histomorphology. Fetal liver cells were used for karyotype analysis. RESULTS: Significant decreases in fetal body weight, and crown-rump length were observed in SNP-treated groups. Exencephaly, small head, scoliosis, lordosis, short thorax and trunk, also fused digits were detected in SNPs-treated groups. Fibrosis, necrosis, and apoptotic cells in fetal midbrains increased significantly in the GD8-14 and GD1-14 groups compared to the control group. Chromosomal features were not different in fetuses between groups. CONCLUSIONS: Exposure to 1 mg/kg/day SNP during pregnancy in mice adversely affected on fetal development. The results do suggest a potential risk for humans that needs to be followed up with more definitive investigations.
BACKGROUND:Silver nanoparticles (SNPs) are being increasingly used in medical and industrial products. The aim of the present study was to evaluate the toxic effect of maternal exposure to SNP (1 mg/kg/day, 70 nm) on fetal development during the first and second weeks of pregnancy. METHODS: Twenty-four pregnant mice were divided into four groups. SNP was administered by oral gavage on gestational days (GD) 1-7, GD8-14, or GD1-14. Phosphate buffered saline was administered by oral gavage to a control group. On GD15, the uteri were excised, and fetal bodies and placentas were weighed. Head and placental circumferences and fetal crown-rump length were measured, and fetuses were evaluated for external malformation. TUNEL assay was performed to assess apoptosis in the fetal midbrain. Hematoxylin-eosin staining was carried out to determine changes in fetal histomorphology. Fetal liver cells were used for karyotype analysis. RESULTS: Significant decreases in fetal body weight, and crown-rump length were observed in SNP-treated groups. Exencephaly, small head, scoliosis, lordosis, short thorax and trunk, also fused digits were detected in SNPs-treated groups. Fibrosis, necrosis, and apoptotic cells in fetal midbrains increased significantly in the GD8-14 and GD1-14 groups compared to the control group. Chromosomal features were not different in fetuses between groups. CONCLUSIONS: Exposure to 1 mg/kg/day SNP during pregnancy in mice adversely affected on fetal development. The results do suggest a potential risk for humans that needs to be followed up with more definitive investigations.