| Literature DB >> 31617177 |
Andrea Vannini1, Biljana Petrovic2, Valentina Gatta1, Valerio Leoni1, Simona Pepe1, Laura Menotti3, Gabriella Campadelli-Fiume4, Tatiana Gianni1.
Abstract
In the previous chapter, we describe the engineering of a HSV-BAC genome by galK recombineering. Here we describe the procedures to reconstitute, or regenerate, the replicating recombinant virus, and the methods to purify it and characterize it for the correct expression of the transgene. We present the example of R-115, a recombinant expressing murine interleukin 12 (mIL12) from the US1-US2 intergenic region. A specific method for the production of highly purified virions by iodixanol gradient, suitable for in vivo applications, is also detailed.Entities:
Keywords: HSV rescue; Plaque assay; Plaque purification; Virion purification; mIL12 transgene expression
Year: 2020 PMID： 31617177 DOI： 10.1007/978-1-4939-9814-2_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745