Literature DB >> 3161411

Isolation of lambda phage DNA by hydroxylapatite chromatography.

I Ivanov, L Gigova.   

Abstract

A simple and rapid (1 day) method for preparation of lambda phage DNA was proposed. The method included two main steps: (a) growth and lysis of bacteria containing lambda phage and (b) purification of lambda phage DNA by hydroxylapatite chromatography. The phage DNA prepared by this method was intact and free of RNA, proteins, and bacterial DNA.

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Year:  1985        PMID: 3161411     DOI: 10.1016/0003-2697(85)90556-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  2 in total

1.  Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli.

Authors:  J E Galán; J F Timoney
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

2.  Cloning and expression in Escherichia coli of a protective antigen of Erysipelothrix rhusiopathiae.

Authors:  J E Galán; J F Timoney
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

  2 in total

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