| Literature DB >> 31614077 |
Priyan D Weerappuli1,2, Cameron Louttit1,2, Taisuke Kojima3, Luke Brennan1, Srilakshmi Yalavarthi4, Yao Xu2,5, Lukasz J Ochyl2,5, Midori L Maeda3, Hong Sun Kim1, Jason S Knight4, Shuichi Takayama3, James J Moon1,2,5.
Abstract
Extracellular traps (ETs), such as neutrophil extracellular traps, are a physical mesh deployed by immune cells to entrap and constrain pathogens. ETs are immunogenic structures composed of DNA, histones, and an array of variable protein and peptide components. While much attention has been paid to the multifaceted function of these structures, mechanistic studies of ETs remain challenging due to their heterogeneity and complexity. Here, a novel DNA-histone mesostructure (DHM) formed by complexation of DNA and histones into a fibrous mesh is reported. DHMs mirror the DNA-histone structural frame of ETs and offer a facile platform for cell culture studies. It is shown that DHMs are potent activators of dendritic cells and identify both the methylation state of DHMs and physical interaction between dendritic cells and DHMs as key tuning switches for immune stimulation. Overall, the DHM platform provides a new opportunity to study the role of ETs in immune activation and pathophysiology.Entities:
Keywords: DNA; extracellular traps; histones; immunostimulation; methylation
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Year: 2019 PMID： 31614077 PMCID： PMC6872909 DOI： 10.1002/adhm.201900926
Source DB: PubMed Journal: Adv Healthc Mater ISSN： 2192-2640 Impact factor: 9.933